Evidence map›Paper›PMID 32994224›Full record

ArticleThe Journal of biological chemistry2020

A saturating mutagenesis CRISPR-Cas9-mediated functional genomic screen identifies

Matthew C Canver, Pratibha Tripathi, Michael J Bullen, Moshe Olshansky, Yogesh Kumar, Lee H Wong, Stephen J Turner, Samuel Lessard, Luca Pinello, Stuart H Orkin and 1 more

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of biological chemistry, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
0.7field-weighted citation impact, top 32% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 13 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Article
  6. Review
  7. Review
  8. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 7 institutions in 3 countries.

Matthew C CanverDivision of Hematology/Oncology, Boston Children's Hospital and Department of Pediatric Oncology, Dana-Farber Cancer Institute (DFCI), Harvard Stem Cell Institute, Harvard Medical School, Boston, Massachusetts, USA.
Pratibha TripathiDepartment of Anatomy and Developmental Biology, Monash University, Clayton, Victoria, Australia; Development and Stem Cells Program, Monash Biomedicine Discovery Institute, Monash University, Clayton, Victoria, Australia.
Michael J BullenDepartment of Anatomy and Developmental Biology, Monash University, Clayton, Victoria, Australia; Development and Stem Cells Program, Monash Biomedicine Discovery Institute, Monash University, Clayton, Victoria, Australia.
Moshe OlshanskyDepartment of Microbiology, Monash Biomedicine Discovery Institute, Monash University, Clayton, Victoria, Australia; Computational Biology and Bioinformatics, Baker Heart and Diabetes Institute, Melbourne, Victoria, Australia.
Yogesh KumarDepartment of Anatomy and Developmental Biology, Monash University, Clayton, Victoria, Australia; Development and Stem Cells Program, Monash Biomedicine Discovery Institute, Monash University, Clayton, Victoria, Australia.
Lee H WongDepartment of Biochemistry and Molecular Biology, Cancer Program, Monash Biomedicine Discovery Institute, Monash University, Clayton, Victoria, Australia.
Stephen J TurnerDepartment of Microbiology, Monash Biomedicine Discovery Institute, Monash University, Clayton, Victoria, Australia; Department of Microbiology and Immunology, University of Melbourne, Parkville, Victoria, Australia.
Samuel LessardResearch Center, Montreal Heart Institute, Montréal, Quebec, Canada; Department of Medicine, Faculty of Medicine, Université de Montréal, Montréal, Quebec, Canada.
Luca PinelloMolecular Pathology and Cancer Center, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, USA.
Stuart H OrkinDivision of Hematology/Oncology, Boston Children's Hospital and Department of Pediatric Oncology, Dana-Farber Cancer Institute (DFCI), Harvard Stem Cell Institute, Harvard Medical School, Boston, Massachusetts, USA; Howard Hughes Medical Institute, Boston, Massachusetts, USA. Electronic address: stuart_orkin@dfci.harvard.edu.
Partha Pratim DasDepartment of Anatomy and Developmental Biology, Monash University, Clayton, Victoria, Australia; Development and Stem Cells Program, Monash Biomedicine Discovery Institute, Monash University, Clayton, Victoria, Australia. Electronic address: partha.das@monash.edu.
Discovery Institute · USBoston Children's Hospital · USBaker Heart and Diabetes Institute · AUHarvard University · USMonash University · AUMontreal Heart Institute · CAThe University of Melbourne · AU

Funding

Multiscale exploration of the functional non-coding genomeR35HG010717 · NHGRI · MASSACHUSETTS GENERAL HOSPITAL · PI PINELLO, LUCA · 2019 to 2023
$2.6M
Discovering disease-causal variants by linking genetic and epigenetic variationR00HG008399 · NHGRI · MASSACHUSETTS GENERAL HOSPITAL · PI PINELLO, LUCA · 2017 to 2019
$747k
Howard Hughes Medical InstituteNHGRI NIH HHS R00 HG008399NHGRI NIH HHS R35 HG010717
6 · The paper itself

Abstract

Regulatory elements (REs) consist of enhancers and promoters that occupy a significant portion of the noncoding genome and control gene expression programs either in

Indexed as

Genetic LociRegulatory Elements, TranscriptionalAnimalsCell LineCRISPR-Cas SystemsGenome-Wide Association StudyHumansMiceMouse Embryonic Stem CellsOctamer Transcription Factor-3Octamer Transcription Factor-3Pou5f1 protein, mouseCRISPR/Cas9ctSCAN-SMSembryonic stem cellfunctional genomicsgene regulationregulatory elements (REs)transcription enhancer

Identifiers

PMID32994224
PMCPMC7681025
OpenAlexW3089940525

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.