ArticleTicks and tick-borne diseases2020
Evaluation of a novel multiplex PCR amplicon sequencing assay for detection of human pathogens in Ixodes ticks.
Article in Ticks and tick-borne diseases, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 19 citations in OpenAlex.
- Efficacy of a low-dose fipronil bait in controlling blacklegged tick (Ixodes scapularis) larvae parasitizing white-footed mice (Peromyscus leucopus) under field conditions in the northeastern United States.Parasites & vectors · 2026Article
- Southward Expansion of Ixodes scapularis (Blacklegged) Tick Pathogens - Western North Carolina, November 2024-August 2025.MMWR. Morbidity and mortality weekly report · 2026Article
- Notes from the Field: Borrelia mayonii Lyme Disease - New York, 2025.MMWR. Morbidity and mortality weekly report · 2026Article
- A CRISPR/LbCas12a system for Borrelia burgdorferi sensu stricto detection in blacklegged ticks.Journal of medical entomology · 2026Article
- Estimated density of Borrelia burgdorferi sensu stricto-infected Ixodes scapularis nymphs in the eastern United States.Parasites & vectors · 2025Article
- A NovelPathogens (Basel, Switzerland) · 2025Article
- Cattle Zoonotic and Non-Zoonotic Tick-Borne Pathogens in Europe-A Retrospective Analysis of the Past 15 Years.Animals : an open access journal from MDPI · 2025Review
- Acquisition of Borrelia burgdorferi sensu stricto (Spirochaetales: Spirochaetaceae) by Haemaphysalis longicornis (Acari: Ixodidae) nymphs during interrupted feeding.Journal of medical entomology · 2025Article
- Geographic variation in the distribution of Anaplasma phagocytophilum variants in host-seeking Ixodes scapularis nymphs and adults in the eastern United States elucidated using next generation sequencing.Ticks and tick-borne diseases · 2024Article
- A next generation sequencing assay combining Ixodes species identification with pathogen detection to support tick surveillance efforts in the United States.Ticks and tick-borne diseases · 2024Article
- Detection of Borrelia burgdorferi sensu lato species in host-seeking Ixodes species ticks in the United States.Ticks and tick-borne diseases · 2024Article
- Ticks and tick-borne microbes identified through passive and active surveillance in Alaska.Journal of medical entomology · 2023Article
- A bioinformatics pipeline for a tick pathogen surveillance multiplex amplicon sequencing assay.Ticks and tick-borne diseases · 2023Article
- Development of a quadruplex PCR amplicon next generation sequencing assay for detection and differentiation ofFrontiers in microbiology · 2023Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Tickborne diseases are an increasing public health concern in the United States, where the majority of notifiable cases are caused by pathogens vectored by Ixodes ticks. To better monitor changes in acarological risk of human encounters with these ticks and their associated pathogens, the Centers for Disease Control and Prevention (CDC) recently established a national tick and tickborne pathogen surveillance program. Here, we describe and evaluate a new Multiplex PCR Amplicon Sequencing (MPAS) assay for potential use in surveillance programs targeting two common human-biting vector ticks, Ixodes scapularis and Ixodes pacificus. The ability of the MPAS assay to detect five Ixodes-associated human pathogens (Borrelia burgdorferi sensu stricto, Borrelia mayonii, Borrelia miyamotoi, Anaplasma phagocytophilum and Babesia microti) was compared to that of a previously published and routinely used probe-based (TaqMan) PCR testing algorithm for pathogen detection in Ixodes ticks. Assay performance comparisons included a set of 175 host-seeking Ixodes nymphs collected in Connecticut as well as DNA from our pathogen reference collection. The MPAS assay and the CDC standard TaqMan PCR pathogen testing algorithm were found to have equivalent detection sensitivity for Ixodes-associated human pathogens. However, the MPAS assay was able to detect a broader range of tick-associated microorganisms, more effectively detected co-infections of multiple pathogens in a single tick (including different species within the Borrelia burgdorferi sensu lato complex), and required a smaller volume of test sample (thus preserving more sample for future testing).
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Registered trials
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