ArticleParasites & vectors2020
Epidemiology and genotyping of Anaplasma marginale and co-infection with piroplasms and other Anaplasmataceae in cattle and buffaloes from Egypt.
Article in Parasites & vectors, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers, 3 of them syntheses that pooled it.
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Who cites it
32 citing papers in PubMed, 3 syntheses or guidelines pooled it, 53 citations in OpenAlex.
- Anaplasma platys beyond canines: a systematic review of host range, phylogenetic relatedness, and knowledge gaps in Africa.Parasites & vectors · 2026Pooled it
- Systematic Review and Meta-Analysis on Piroplasma spp. Infection and Co-infection with Anaplasma marginale in Domestic Ruminants from Algeria.Acta parasitologica · 2024Pooled it
- Global prevalence and species diversity of tick-borne pathogens in buffaloes worldwide: a systematic review and meta-analysis.Parasites & vectors · 2023Pooled it
- An updated assessment of the molecular prevalence and risk factors of Babesia infection among crossbred cattle: a diagnostic cross-sectional study.BMC veterinary research · 2026Article
- Nomenclature, classification, and in-silico structural analysis of MSP1a variants of Anaplasma marginale.PloS one · 2026Article
- Significance of molecular and zymography profiling for accurate detection of infected sheep with Anaplasma marginale in South Sinai, Egypt.Scientific reports · 2025Article
- Molecular Characterization of Tick-Borne Pathogens in Jiangxi Province: A High Prevalence ofPathogens (Basel, Switzerland) · 2025Article
- Temporal changes in the bacterial microbiome of the salivary gland and midgut tissues of Rhipicephalus sanguineus (s.l.) ticks in South Africa.Scientific reports · 2025Article
- High Diversity and Prevalence of Rickettsial Agents inMicroorganisms · 2025Article
- Molecular detection ofJournal of parasitic diseases : official organ of the Indian Society for Parasitology · 2024Article
- Molecular screening and genetic diversity of tick-borne pathogens associated with dogs and livestock ticks in Egypt.PLoS neglected tropical diseases · 2024Article
- Isolation and propagation of an Egyptian Theileria annulata infected cell line and evaluation of its use as a vaccine to protect cattle against field challenge.Scientific reports · 2024Article
- Meta-analysis of tick-borne and other pathogens: Co-infection or co-detection? That is the question.Current research in parasitology & vector-borne diseases · 2024Review
- Molecular prevalence ofVeterinary world · 2023Article
- Review
- Molecular detection and characterization ofFrontiers in veterinary science · 2023Article
- Residue Depletion of Imidocarb in Bovine Tissues by UPLC-MS/MS.Animals : an open access journal from MDPI · 2022Article
- Tick-borne diseases in Egypt: A one health perspective.One health (Amsterdam, Netherlands) · 2022Review
- Detecting zoonotic and non-zoonotic pathogens in livestock and their ticks in Corsican wetlands.Veterinary medicine and science · 2022Article
- Molecular Epidemiological Investigation of Piroplasms and Anaplasmataceae Bacteria in Egyptian Domestic Animals and Associated Ticks.Pathogens (Basel, Switzerland) · 2022Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
backgroundAnaplasma marginale is an obligate intracellular bacterium and the main cause of bovine anaplasmosis in tropical and subtropical regions. In Egypt, data regarding the prevalence of A. marginale in ruminant hosts and of the circulating genotypes is lacking. This study therefore aimed to (i) investigate the presence, epidemiology and genotypes of A. marginale in cattle and buffaloes in Egypt, (ii) to evaluate suitable diagnostic tools and (iii) to identify co-infections of A. marginale with other selected tick-borne pathogens.
methodsBlood samples were collected from 394 animals (309 cattle and 85 buffaloes) from three different areas in Egypt. For the detection of A. marginale infection, several tests were compared for their sensitivity and specificity: blood smear analysis, enzyme-linked immunosorbent assay (ELISA), PCR, real-time PCR and reverse line blot (RLB) assay. Co-infections with A. marginale, piroplasms and other Anaplasmataceae were surveyed by RLB while A. marginale genotypes were identified by amplifying and sequencing the partial msp1α gene.
resultsAnaplasma marginale DNA was amplified by qPCR in 68.3% of cattle and 29.4% of buffaloes. RLB showed infection with A. marginale in 50.2% of cattle and 42.5% of buffaloes. Blood smear analysis detected this agent in 16.2% of cattle and 2.4% of buffaloes. ELISA showed specific antibodies against A. marginale in 54.9% of cattle. Anaplasma marginale was associated, in cattle and buffaloes, with several tick-borne pathogens (Theileria annulata, Babesia bovis, Babesia bigemina, Babesia occultans and Anaplasma platys). A significant difference of A. marginale infection level was noticed in cattle, where animals between 3-5-years-old had a higher prevalence (79.2%) compared to those older than 5 years (36.4%) and younger than 3 years (59.7%) and one year (64.5%), respectively (P = 0.002281). Microsatellite analysis identified 15 different genotypes.
conclusionsThe epidemiological findings revealed high prevalence of A. marginale in cattle and buffaloes in all the investigated areas. The circulation of diverse genotypes was observed, most of these A. marginale genotypes being specific for Egypt. The qPCR assay was confirmed to be the most sensitive tool for detection of A. marginale in cattle and buffaloes even in the carrier state, highlighting the importance of using suitable diagnostic tests.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.