Evidence map›Paper›PMID 32993477›Full record

ArticleBMC genomics2020

Identification of Y chromosome markers in the eastern three-lined skink (Bassiana duperreyi) using in silico whole genome subtraction.

Duminda Sampath Bandara Dissanayake, Clare Ellen Holleley, Laura Kate Hill, Denis O'Meally, Janine Eileen Deakin, Arthur Georges

Open access · goldAbstract read
In one paragraph

Article in BMC genomics, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
4.1field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed, 31 citations in OpenAlex.

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  11. Sex-specific splicing of Z- and W-borneProceedings of the National Academy of Sciences of the United States of America · 2022
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 2 countries.

Duminda Sampath Bandara DissanayakeInstitute for Applied Ecology, University of Canberra, Canberra, ACT, 2601, Australia.ORCID https://orcid.org/0000-0002-7307-4639
Clare Ellen HolleleyInstitute for Applied Ecology, University of Canberra, Canberra, ACT, 2601, Australia.ORCID https://orcid.org/0000-0002-5257-0019
Laura Kate HillInstitute for Applied Ecology, University of Canberra, Canberra, ACT, 2601, Australia.
Denis O'MeallyInstitute for Applied Ecology, University of Canberra, Canberra, ACT, 2601, Australia.ORCID https://orcid.org/0000-0001-7749-9506
Janine Eileen DeakinInstitute for Applied Ecology, University of Canberra, Canberra, ACT, 2601, Australia.ORCID https://orcid.org/0000-0002-1259-3531
Arthur GeorgesInstitute for Applied Ecology, University of Canberra, Canberra, ACT, 2601, Australia. georges@aerg.canberra.edu.au.ORCID http://orcid.org/0000-0003-2428-0361
University of Canberra · AUCity Of Hope National Medical Center · US

Funding

Australian Research Council DP110104377Australian Research Council DP170101147
6 · The paper itself

Abstract

backgroundHomologous sex chromosomes can differentiate over time because recombination is suppressed in the region of the sex determining locus, leading to the accumulation of repeats, progressive loss of genes that lack differential influence on the sexes and sequence divergence on the hemizygous homolog. Divergence in the non-recombining regions leads to the accumulation of Y or W specific sequence useful for developing sex-linked markers. Here we use in silico whole-genome subtraction to identify putative sex-linked sequences in the scincid lizard Bassiana duperreyi which has heteromorphic XY sex chromosomes.

resultsWe generated 96.7 × 10

conclusionsThe sex of B. duperreyi can be reversed by low temperatures (XX genotype reversed to a male phenotype). We have developed sex-specific markers to identify the underlying genotypic sex and its concordance or discordance with phenotypic sex in wild populations of B. duperreyi. Our pipeline can be applied to isolate Y or W chromosome-specific sequences of any organism and is not restricted to sequence residing within single-copy genes. This study greatly improves our knowledge of the Y chromosome in B. duperreyi and will enhance future studies of reptile sex determination and sex chromosome evolution.

Indexed as

AnimalsComputer SimulationFemaleGenetic LociGenotyping TechniquesLizardsMaleWhole Genome SequencingY ChromosomeGenotypic sex determinationSex reversalSex-specific markersY chromosome

Identifiers

PMID32993477
PMCPMC7526180
OpenAlexW3090561484

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.