ArticlePlant & cell physiology2020
Potato Virus X Vector-Mediated DNA-Free Genome Editing in Plants.
Article in Plant & cell physiology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
53 citing papers in PubMed, 109 citations in OpenAlex.
- Genome Editing in Solanaceae: Harnessing CRISPR-Cas Technology for Precision Crop Improvement.Plants (Basel, Switzerland) · 2026Review
- A Modified Cas9 Scaffold Allows Extension of the Virus-Induced Gene Editing Technology to the Large Potyvirus Genus.Plant biotechnology journal · 2026Article
- Developing a mobile RNA delivery system for grafting trait improvement.EMBO reports · 2026Article
- Efficient CRISPR-Cas9 delivery and transgene-free multiplex genome editing in plants using cymbidium mosaic virus-derived vectors.The Plant journal : for cell and molecular biology · 2026Article
- Targeted mutagenesis and base editing using engineeredPlant biotechnology (Tokyo, Japan) · 2026Article
- Plant viruses as next-generation vectors for transgene-free genome editing, gene regulation, and rapid crop improvement.Plant molecular biology · 2026Review
- Virus-induced genome editing enables functional genomics across diverse plant species.Molecules and cells · 2026Review
- Virus-Mediated System for Simultaneous Gene Silencing and Genome Editing in Cotton.Plants (Basel, Switzerland) · 2026Article
- Heritable Tissue-Culture-Free Gene Editing in Nicotiana benthamiana Using a Meristem-Invading Virus Vector.Methods in molecular biology (Clifton, N.J.) · 2026Article
- CRISPR/Cas system-mediated transgene-free or DNA-free genome editing in plants.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Review
- Virus-induced gene editing free from tissue culture.Nature plants · 2025Review
- Virus-induced systemic and heritable gene editing in pepper (Capsicum annuum L.).The Plant journal : for cell and molecular biology · 2025Article
- Virus-Induced Genome Editing (VIGE): One Step Away from an Agricultural Revolution.International journal of molecular sciences · 2025Review
- Viral delivery of an RNA-guided genome editor for transgene-free germline editing in Arabidopsis.Nature plants · 2025Article
- Development of an efficient and heritable virus-induced genome editing system inHorticulture research · 2025Article
- Enhancing virus-mediated genome editing for cultivated tomato through low temperature.Plant cell reports · 2025Article
- Viral vector-based transient expression systems for plant biotechnology research at PUIs.Frontiers in education · 2025Article
- Editorial: Engineering future crops through genome editing.Frontiers in plant science · 2025Article
- Rapid and efficient in planta genome editing in sorghum using foxtail mosaic virus-mediated sgRNA delivery.The Plant journal : for cell and molecular biology · 2025Article
- Heritable Tissue-Culture-Free Gene Editing in Nicotiana benthamiana through Viral Delivery of SpCas9 and sgRNA.Plant & cell physiology · 2024Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Genome editing technology is important for plant science and crop breeding. Genome-edited plants prepared using general CRISPR-Cas9 methods usually contain foreign DNA, which is problematic for the production of genome-edited transgene-free plants for vegetative propagation or highly heterozygous hybrid cultivars. Here, we describe a method for highly efficient targeted mutagenesis in Nicotiana benthamiana through the expression of Cas9 and single-guide (sg)RNA using a potato virus X (PVX) vector. Following Agrobacterium-mediated introduction of virus vector cDNA, >60% of shoots regenerated without antibiotic selection carried targeted mutations, while ≤18% of shoots contained T-DNA. The PVX vector was also used to express a base editor consisting of modified Cas9 fused with cytidine deaminase to introduce targeted nucleotide substitution in regenerated shoots. We also report exogenous DNA-free genome editing by mechanical inoculation of virions comprising the PVX vector expressing Cas9. This simple and efficient virus vector-mediated delivery of CRISPR-Cas9 could facilitate transgene-free gene editing in plants.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.