ArticleAnalytical and bioanalytical chemistry2020
Lipidomic profiling of single mammalian cells by infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI).
Article in Analytical and bioanalytical chemistry, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- The MALDI Method to Analyze the Lipid Profile, Including Cholesterol, Triglycerides and Other Lipids.Current issues in molecular biology · 2026Review
- Distinguishing the Metabolic Effect of Fetal and Adult Fibrinogen on Human Fibroblast Cell Culture by IR-MALDESI Mass Spectrometry Imaging.Journal of the American Society for Mass Spectrometry · 2025Article
- Recent Developments in Single-Cell Metabolomics by Mass Spectrometry─A Perspective.Journal of proteome research · 2025Review
- Applications of MALDI mass spectrometry in forensic science.Analytical and bioanalytical chemistry · 2024Review
- Optimizing neurotransmitter pathway detection by IR-MALDESI-MSI in mouse brain.Analytical and bioanalytical chemistry · 2024Article
- The Chemical Reactivity of Membrane Lipids.Chemical reviews · 2024Review
- Advances in multimodal mass spectrometry for single-cell analysis and imaging enhancement.FEBS letters · 2024Review
- Mass Spectrometry-based Single-Cell Lipidomics: Advancements, Challenges, and the Path Forward.Trends in analytical chemistry : TRAC · 2023Article
- Analytical Techniques for Single-Cell Biochemical Assays of Lipids.Annual review of biomedical engineering · 2023Review
- Novel matrix strategies for improved ionization and spatial resolution using IR-MALDESI mass spectrometry imaging.Journal of mass spectrometry : JMS · 2022Article
- Mass Spectrometry Imaging ofJournal of proteome research · 2022Article
- Enhancing Metabolomic Coverage in Positive Ionization Mode Using Dicationic Reagents by Infrared Matrix-Assisted Laser Desorption Electrospray Ionization.Metabolites · 2021Article
- Spatially resolved metabolomic characterization of muscle invasive bladder cancer by mass spectrometry imaging.Metabolomics : Official journal of the Metabolomic Society · 2021Article
- Single-cell omics analyses with single molecular detection: challenges and perspectives.Journal of biomedical research · 2021Article
- Spatially Resolved Mass Spectrometry at the Single Cell: Recent Innovations in Proteomics and Metabolomics.Journal of the American Society for Mass Spectrometry · 2021Review
- Applications of ambient ionization mass spectrometry in 2020: An annual review.Analytical science advances · 2021Review
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3 authors.
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Abstract
To better understand cell-to-cell heterogeneity, advanced analytical tools are in a growing demand for elucidating chemical compositions of each cell within a population. However, the progress of single-cell chemical analysis has been restrained by the limitations of small cell volumes and minute cellular concentrations. Here, we present a rapid and sensitive method for investigating the lipid profiles of isolated single cells using infrared matrix-assisted laser desorption electrospray ionization mass spectrometry (IR-MALDESI-MS). In this work, HeLa cells were dispersed onto a glass slide, and the cellular contents were ionized by IR-MALDESI and measured using a Q-Exactive HF-X mass spectrometer. Importantly, this approach does not require extraction and/or enrichment of analytes prior to MS analysis. Using this approach, 45 distinct lipid species, predominantly phospholipids, were detected and putatively annotated from the single HeLa cells. The proof-of-concept study demonstrates the feasibility and efficacy of IR-MALDESI-MS for rapid lipidomic profiling of single cells, which provides an important basis for future work on differentiation between normal and diseased cells at various developmental states, which can offer new insights into cellular metabolic pathways and pathological processes. Although not yet accomplished, we believe this approach can be readily used as an assessment tool to compare the number of identified species during source evolution and method optimization (intra-laboratory), and also disclose the complementary nature of different direct analytical approaches for the coverage of different types of endogenous analytes (inter-laboratory).Graphical abstract.
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