ArticleNature methods2020
An optimized acetylcholine sensor for monitoring in vivo cholinergic activity.
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Article in Nature methods, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 249 papers.
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249citing papers in PubMed
18.0field-weighted citation impact, top 1% of its field
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Who cites it
249 citing papers in PubMed, 433 citations in OpenAlex.
- A Chemigenetic Fluorescence Lifetime Biosensor for Dopamine Sensing.bioRxiv : the preprint server for biology · 2026Article
- Modulatory effects of α7-nicotinic cholinergic receptors on perceptual sensitivity in a visual signal detection task.Psychopharmacology · 2026Article
- Cholinergic-dependent dopamine signals in mouse dorsomedial striatum are regulated by frontal but not sensory cortices.Nature communications · 2026Article
- Acetylcholine: a candidate substrate for hippocampal predictive learning?Nature reviews. Neuroscience · 2026Review
- Protein Surface Site Determines the Evolutionary Accessibility of Allosteric Regulation.bioRxiv : the preprint server for biology · 2026Article
- 5-HT4 Receptor Ligand RS67333 Modulates Striatal Acetylcholine and Dopamine Release via the Inhibition of Acetylcholinesterase.ACS chemical neuroscience · 2026Article
- Article
- Striatal control of amygdalar acetylcholine release during salience-associated processing.Nature neuroscience · 2026Article
- Red-shifted GRAB acetylcholine sensors for multiplex imaging in vivo.Nature neuroscience · 2026Article
- Early-Stage Corticostriatal Hyperactivity Impairs Cognitive Flexibility Alongside Striatal Cholinergic Dysfunction in an Alzheimer's Disease Model.Nature communications · 2026Article
- Cholinergic interneuron control of GABAergic circuits targeting spiny projection neurons is disrupted in parkinsonian models.bioRxiv : the preprint server for biology · 2026Article
- A synaptic mechanism for encoding the learned value of action-derived safety.Nature communications · 2026Article
- Spontaneous and stimulus-driven arousal produce distinct acetylcholine dynamics across sensory and prefrontal cortex.bioRxiv : the preprint server for biology · 2026Article
- Automated device for permitting free movement during simultaneous photometry and electrophysiology in mice.Nature methods · 2026Article
- Fast analysis and engineering of protein function by microbe-independent deep assembly and screening.Molecular systems biology · 2026Article
- Neuromodulator Dynamics Underlying Associative Learning in the Ventral Striatum's Olfactory Tubercle.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- A highly sensitive genetically encoded red cAMP sensor for multiplex imaging in vivo.Nature communications · 2026Article
- Thalamus orchestrates local acetylcholine-dependent dopamine release in the learning striatum.bioRxiv : the preprint server for biology · 2026Article
- Spatiotemporally dynamic noradrenergic regulation of cortical networks.bioRxiv : the preprint server for biology · 2026Article
- A Novel Acetylcholine Nanosensor for Single Vesicle Storage and Sub-Quantal Exocytosis in Living Neurons and Organoids.Angewandte Chemie (International ed. in English) · 2026Article
189 more citing papers are in PubMed but not listed here.
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Authors and funding
24 authors at 8 institutions in 3 countries.
Miao JingChinese Institute for Brain Research, Beijing, China. jingmiao@cibr.ac.cn.ORCID http://orcid.org/0000-0002-7782-5065
Yuexuan LiState Key Laboratory of Membrane Biology, Peking University School of Life Sciences, Beijing, China.ORCID http://orcid.org/0000-0001-6099-8288
Jianzhi ZengState Key Laboratory of Membrane Biology, Peking University School of Life Sciences, Beijing, China.ORCID http://orcid.org/0000-0002-5380-6281
Pengcheng HuangBritton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics; MoE Key Laboratory for Biomedical Photonics, Collaborative Innovation Center for Biomedical Engineering, School of Engineering Sciences, Huazhong University of Science and Technology, Wuhan, China.
Miguel SkirzewskiBrainsCAN Rodent Cognition Core, The University of Western Ontario, London, Ontario, Canada.
Ornela KljakicRobarts Research Institute, Department of Physiology and Pharmacology, Schulich School of Medecine and Dentistry, Brain and Mind Institute, The University of Western Ontario, London, Ontario, Canada.
Wanling PengInstitute of Neuroscience, State Key Laboratory of Neuroscience, CAS Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, Shanghai, China.
Tongrui QianState Key Laboratory of Membrane Biology, Peking University School of Life Sciences, Beijing, China.
Ke TanState Key Laboratory of Membrane Biology, Peking University School of Life Sciences, Beijing, China.
Jing ZouDepartment of Biological Sciences, Section of Neurobiology, University of Southern California, Los Angeles, CA, USA.
Simon TrinhDepartment of Biological Sciences, Section of Neurobiology, University of Southern California, Los Angeles, CA, USA.ORCID http://orcid.org/0000-0003-0125-5016
Runlong WuInstitute of Molecular Medicine, Peking University, Beijing, China.
Shichen ZhangState Key Laboratory of Membrane Biology, Peking University School of Life Sciences, Beijing, China.
Sunlei PanState Key Laboratory of Membrane Biology, Peking University School of Life Sciences, Beijing, China.
Samuel A HiresDepartment of Biological Sciences, Section of Neurobiology, University of Southern California, Los Angeles, CA, USA.
Min XuInstitute of Neuroscience, State Key Laboratory of Neuroscience, CAS Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, Shanghai, China.ORCID http://orcid.org/0000-0002-6936-7543
Haohong LiBritton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics; MoE Key Laboratory for Biomedical Photonics, Collaborative Innovation Center for Biomedical Engineering, School of Engineering Sciences, Huazhong University of Science and Technology, Wuhan, China.
Lisa M SaksidaRobarts Research Institute, Department of Physiology and Pharmacology, Schulich School of Medecine and Dentistry, Brain and Mind Institute, The University of Western Ontario, London, Ontario, Canada.
Vania F PradoRobarts Research Institute, Department of Physiology and Pharmacology, Schulich School of Medecine and Dentistry, Brain and Mind Institute, The University of Western Ontario, London, Ontario, Canada.ORCID http://orcid.org/0000-0003-4994-6393
Timothy J BusseyRobarts Research Institute, Department of Physiology and Pharmacology, Schulich School of Medecine and Dentistry, Brain and Mind Institute, The University of Western Ontario, London, Ontario, Canada.ORCID http://orcid.org/0000-0002-3180-3709
Marco A M PradoRobarts Research Institute, Department of Physiology and Pharmacology, Schulich School of Medecine and Dentistry, Brain and Mind Institute, The University of Western Ontario, London, Ontario, Canada.
Liangyi ChenState Key Laboratory of Membrane Biology, Peking University School of Life Sciences, Beijing, China.ORCID http://orcid.org/0000-0003-1270-7321
Heping ChengState Key Laboratory of Membrane Biology, Peking University School of Life Sciences, Beijing, China.ORCID http://orcid.org/0000-0002-9604-6702
Yulong LiState Key Laboratory of Membrane Biology, Peking University School of Life Sciences, Beijing, China. yulongli@pku.edu.cn.ORCID http://orcid.org/0000-0002-9166-9919
Peking University · CNWestern University · CAUniversity of Southern California · USChinese Academy of Sciences · CNMcGovern Institute for Brain Research · USWuhan National Laboratory for Optoelectronics · CNChinese Academy of Medical Sciences & Peking Union Medical College · CNChinese Institute for Brain Research · CN
Funding
Research Supplement to Promote Diversity in Health-Related ResearchR01MH101377 · NIMH · NEW YORK UNIVERSITY SCHOOL OF MEDICINE · PI Dayu Lin · 2013 to 2026
$8.6MNovel fluorescent sensors for imaging neuromodulationU01NS113358 · NINDS · UNIVERSITY OF CALIFORNIA BERKELEY · PI DAN, YANG, LI, YULONG · 2019 to 2022
$4.3MNovel fluorescent sensors based on GPCRs for imaging neuromodulationU01NS103558 · NINDS · UNIVERSITY OF SOUTHERN CALIFORNIA · PI HIRES, SAMUEL ANDREW, LI, YULONG · 2017 to 2019
$2.2MCIHR PJT 159781NIMH NIH HHS R01 MH101377NINDS NIH HHS U01 NS103558NINDS NIH HHS U01 NS113358
Abstract
The ability to directly measure acetylcholine (ACh) release is an essential step toward understanding its physiological function. Here we optimized the GRAB
Indexed as
AcetylcholineAnimalsAnimals, Genetically ModifiedBehavior, AnimalBiosensing TechniquesBrainCholinergic AgentsDrosophilaGreen Fluorescent ProteinsHEK293 CellsHumansMiceMushroom BodiesNeuronsOlfactory CortexReceptor, Muscarinic M3AcetylcholineCholinergic AgentsGreen Fluorescent ProteinsReceptor, Muscarinic M3
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.