ArticlePoultry science2020
Antibacterial and antibiofilm activities of thyme oil against foodborne multiple antibiotics-resistant Enterococcus faecalis.
Article in Poultry science, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 48 citations in OpenAlex.
- From Plant-Derived Compound to Bioactive Agent in Dentistry: The Expanding Role of Thymol.Dentistry journal · 2026Review
- Antibacterial and anti-biofilm activities of thyme oil, oregano oil, and their combination against Klebsiella pneumoniae and Acinetobacter baumannii polymicrobial biofilms.Antonie van Leeuwenhoek · 2026Article
- Enhanced antibiofilm and gene-suppressive effects of cinnamon nanoemulsion against multidrug-resistant Enterococcus faecalis.Molecular biology reports · 2026Article
- Thyme essential oil potentials as a bactericidal and biofilm-preventive agent against prevalent bacterial pathogens.Scientific reports · 2025Article
- Essential Oils for Biofilm Control: Mechanisms, Synergies, and Translational Challenges in the Era of Antimicrobial Resistance.Antibiotics (Basel, Switzerland) · 2025Review
- In vitro antibacterial and antibiofilm activities of isobavachalcone against Enterococcus faecalis clinical isolates from China.BMC microbiology · 2025Article
- Surfactin inhibits enterococcal biofilm formation via interference with pilus and exopolysaccharide biosynthesis.BMC microbiology · 2025Article
- Contribution of Phosphorylation Modification to Stability and Antibacterial Activity of Egg White Protein Nanogels Loaded with Cinnamon Bark Essential Oil.Gels (Basel, Switzerland) · 2024Article
- Antibacterial and Antibiofilm Activity of Essential Oils AgainstAnimals : an open access journal from MDPI · 2024Article
- Emerging Approaches for Mitigating Biofilm-Formation-Associated Infections in Farm, Wild, and Companion Animals.Pathogens (Basel, Switzerland) · 2024Review
- Contemporary strategies and approaches for characterizing composition and enhancing biofilm penetration targeting bacterial extracellular polymeric substances.Journal of pharmaceutical analysis · 2024Review
- Effects of essential oils on economically important characteristics of ruminant species: A comprehensive review.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2024Review
- Antibacterial and Antibiofilm Efficacy and Mechanism of Ginger (Plants (Basel, Switzerland) · 2023Article
- Antibacterial Activity and Antibacterial Mechanism of Lemon Verbena Essential Oil.Molecules (Basel, Switzerland) · 2023Article
- Antimicrobial and Antibiofilm Potential ofAntibiotics (Basel, Switzerland) · 2023Article
- Beyond the Risk of Biofilms: An Up-and-Coming Battleground of Bacterial Life and Potential Antibiofilm Agents.Life (Basel, Switzerland) · 2023Review
- The Anti-Biofilm Activity and Mechanism of Apigenin-7-O-Glucoside AgainstInfection and drug resistance · 2023Article
- Effect ofHeliyon · 2022Review
- The Antimicrobial and Antibiofilm Activity of Oregano Essential Oil againstFoods (Basel, Switzerland) · 2022Article
- The Antimicrobial Effect ofFrontiers in microbiology · 2022Article
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The inhibitory and bactericidal activities of thyme oil against the foodborne multiple antibiotics-resistant Enterococcus faecalis biofilm were evaluated in this study. Gas chromatography-mass spectrometry revealed that more than 70% of the composition of thyme oil is thymol. Crystal violet staining assay showed that 128 and 256 μg/mL thyme oil significantly inhibited the biofilm formation of E. faecalis. The cell adherence of E. faecalis, as shown by its swimming and swarming motilities, was reduced by thyme oil. The exopolysaccharide (EPS) quantification assay showed that thyme oil inhibited the EPS synthesis in E. faecalis biofilms. The 3D-view observations through confocal laser scanning and scanning electron microscopy suggested that cell adherence and biofilm thickness were decreased in thyme oil-treated biofilms. Quantitative real-time analyses showed that the transcription of ebp and epa gene clusters, which were related to cell mobility and EPS production, was inhibited by thyme oil. Thus, thyme oil effectively inhibited the biofilm formation of E. faecalis by affecting cell adherence and EPS synthesis. Furthermore, 2,048 and 4,096 μg/mL thyme oil can effectively inactivate E. faecalis population in the mature E. faecalis biofilms by 5.75 and 7.20 log CFU/mL, respectively, after 30 min of treatment. Thus, thyme oil at different concentrations can be used as an effective antibiofilm or germicidal agent to control E. faecalis biofilms.
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