ArticlePoultry science2020
Growth performance and amino acid digestibility responses of broiler chickens fed diets containing purified soybean trypsin inhibitor and supplemented with a monocomponent protease.
Article in Poultry science, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 38 citations in OpenAlex.
- Vanillin Improves Antioxidant Capacity, Immunity, and Gut Microbiota in Broilers.Veterinary sciences · 2026Article
- Investigation of interactions between phytase, protease, and anti-nutritional factors in soybeans.Poultry science · 2026Article
- Mechanism of fumonisin B1 on growth performance and intestinal structural integrity of juvenile grass carp (Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2025Article
- Optimizing the zymogram of exogenous proteases in broiler diets by in vitro simulated gastrointestinal digestion and response surface methodology.Scientific reports · 2025Article
- Technical note: optimizing sample size for broiler chicken and pig intestinal histomorphometry and prediction equations.Journal of animal science · 2025Article
- Rye (Frontiers in nutrition · 2025Review
- Food Additives Derived from Fruits and Vegetables for Sustainable Animal Production and Their Impact in Latin America: An Alternative to the Use of Antibiotics.Foods (Basel, Switzerland) · 2024Review
- Effect of different doses of camelina cake inclusion as a substitute of dietary soyabean meal on growth performance and gut health of weaned pigs.The British journal of nutrition · 2024Article
- Multiprotease improves amino acid release in vitro, energy, and nutrient utilization in broilers fed diets varying in crude protein levels.Poultry science · 2024Article
- Comparative effects of proteases on performance, carcass traits and gut structure of broilers fed diets reduced in protein and amino acids.Journal of animal science and technology · 2024Article
- Dietary supplementation with mushroom powder (Agaricus bisporus) on performance, carcass traits, meat quality, and bone biomechanical properties of quail (Coturnix coturnix japonica).Tropical animal health and production · 2024Article
- Wide-genome selection of lactic acid bacteria harboring genes that promote the elimination of antinutritional factors.Frontiers in plant science · 2023Article
- Amino Acids Supplementation Affects Sustainability of Productive and Meat Quality, Survivability and Nitrogen Pollution of Broiler Chickens during the Early Life.Life (Basel, Switzerland) · 2022Article
- Inactivation of Soybean Trypsin Inhibitor by Dielectric-Barrier Discharge Plasma and Its Safety Evaluation and Application.Foods (Basel, Switzerland) · 2022Article
- Efficiency of Soybean Products in Broiler Chicken Nutrition.Animals : an open access journal from MDPI · 2022Article
- Contribution of purified soybean trypsin inhibitor and exogenous protease to endogenous amino acid losses and mineral digestibility.Poultry science · 2021Article
- Progress on Gut Health Maintenance and Antibiotic Alternatives in Broiler Chicken Production.Frontiers in nutrition · 2021Review
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Trypsin inhibitors (TI) resident in soybeans affects protein utilization. While heat treatment influences residual TI, it simultaneously affects the structure and solubility of the soybean proteins and confounds any response to exogenous proteases. Using purified TI, the effect of exogenous protease to TI can be dissociated from changes in the soybean protein. Thus, the current study was designed to evaluate the growth performance and protein utilization responses of broiler chickens to purified TI and exogenous protease. Soybean meal (SBM) was preanalyzed for basal TI (2,996 TIU/g SBM), formulated into nutritionally adequate experimental diets to contain 1,033 TIU/g diet, and purified TI was added at 9,000 TIU/g diet. A total of 320 Cobb-500 broiler chicks were allocated to 4 diets, each with 8 replicate cages and 10 birds per replicate. The experimental diets were arranged as a 2 × 2 factorial with factors being dietary TI (1,033 or 10,033 TIU/g) and exogenous protease (0 or 15,000 PROT/kg). On day 7, 14, and 21 posthatching, protease supplementation improved the BW gain (P < 0.01) and gain to feed ratio (P < 0.05) of birds. On day 14 and 21 posthatching, the relative weight of pancreas increased (P < 0.05) with added TI but was reduced (P < 0.001) with protease supplementation. Apparent ileal digestibility of all amino acids, except methionine, decreased (P < 0.001) with added TI but increased (P < 0.05) with protease supplementation. Jejunal MUC-2 was downregulated (P < 0.01) and SCL7A-2 was upregulated (P < 0.05) by protease supplementation. Duodenal trypsin and chymotrypsin activities reduced (P < 0.05) with added TI but increased (P < 0.01) with protease supplementation. Exogenous protease produced longer villi (P < 0.05) and deeper crypts (P < 0.01) in the jejunal tissue. In conclusion, dietary addition of purified TI negatively affects nutrient utilization by broiler chickens. Furthermore, the study showed that the efficacy of the exogenous protease might be independent of dietary TI concentration.
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