Evidence map›Paper›PMID 32988516›Full record

ArticlePoultry science2020

Research Note: Rapid detection of avian infectious laryngotracheitis virus with real-time fluorescence-based recombinase-aided amplification.

Wenjing Wang, Chunguang Wang, Zichuang Zhang, Peng Zhang, Shanshan Yao, Jingru Liu, Xianghe Zhai, Tie Zhang

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Poultry science, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.1field-weighted citation impact, top 48% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Wenjing WangCollege of Veterinary Medicine, Hebei Agricultural University, Baoding 071001, China.
Chunguang WangCollege of Veterinary Medicine, Hebei Agricultural University, Baoding 071001, China.
Zichuang ZhangCollege of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, China.
Peng ZhangCollege of Veterinary Medicine, Hebei Agricultural University, Baoding 071001, China.
Shanshan YaoCollege of Veterinary Medicine, Hebei Agricultural University, Baoding 071001, China.
Jingru LiuCollege of Veterinary Medicine, Hebei Agricultural University, Baoding 071001, China.
Xianghe ZhaiCollege of Veterinary Medicine, Hebei Agricultural University, Baoding 071001, China.
Tie ZhangCollege of Veterinary Medicine, Hebei Agricultural University, Baoding 071001, China. Electronic address: zhangtie1998@163.com.
Hebei Agricultural University · CNJiangxi Agricultural University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In this study, specific primers and fluorescent probes were designed to target the thymidine kinase (TK) gene sequence of avian infectious laryngotracheitis virus (ILTV). Through specificity and sensitivity tests, a real-time fluorescence-based recombinase-aided amplification (RF-RAA) method for detecting ILTV was established. The results showed that the method was specific and could be used to accurately detect ILTV, and there was no cross-reaction with Newcastle disease virus (NDV), avian influenza virus (AIV), or infectious bronchitis virus (IBV). Real-time fluorescence-based recombinase-aided amplification had high sensitivity, and the lowest detectable limit (LDL) for ILTV could reach 10 copies/μL, 1,000 times more sensitive than conventional PCR (10

Indexed as

Herpesviridae InfectionsHerpesvirus 1, GallidPoultry DiseasesAnimalsChickensChinaFluorescenceLimit of DetectionNucleic Acid Amplification TechniquesRecombinasesSensitivity and SpecificityRecombinasesinfectious laryngotracheitis virusreal-time fluorescence-based recombinase-aided amplificationTK gene

Identifiers

PMID32988516
PMCPMC7598125
OpenAlexW3038780023

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.