Application of a Flow-Induced Stress Wave and Investigation of Associated Injuries on Cell Monolayers Using a Parallel Plate Flow Chamber.

Samar Shurbaji, Mahmoud Khatib A A Al-Ruweidi, Fatma Hassan Ali, Fatiha M Benslimane, Huseyin C Yalcin

Open access · goldAbstract read

In one paragraph

Article in Methods and protocols, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

0.4field-weighted citation impact, top 33% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 4 citations in OpenAlex.

Dexamethasone and transdehydroandrosterone significantly reduce pulmonary epithelial cell injuries associated with mechanical ventilation.Journal of applied physiology (Bethesda, Md. : 1985) · 2021
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Samar ShurbajiBiomedical Research Center, Qatar University, Doha, P.O. Box 2713, Qatar.

Mahmoud Khatib A A Al-RuweidiBiomedical Research Center, Qatar University, Doha, P.O. Box 2713, Qatar.ORCID 0000-0003-4696-7818

Fatma Hassan AliBiomedical Research Center, Qatar University, Doha, P.O. Box 2713, Qatar.

Fatiha M BenslimaneBiomedical Research Center, Qatar University, Doha, P.O. Box 2713, Qatar.

Huseyin C YalcinBiomedical Research Center, Qatar University, Doha, P.O. Box 2713, Qatar.

Qatar University · QA

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Parallel plate flow chambers are widely used to expose cultured cells to physiological flows for the investigation of a variety of diseases. These applications usually involve the generation of continuous and steady fluid flow over cell monolayers for extended durations, usually a few days. Another technique is to generate a fast high-stress wave over the cells to see the immediate effect of flow-induced stresses. This can be achieved by propagating an air/liquid interface, in other words, a bubble, over cell monolayers. The approach is relevant to the reopening event of fluid-filled lung bronchioles and alveoli during mechanical ventilation therapy of Acute Respiratory Distress Syndrome. This article explains how we generate a stress wave using a parallel plate flow chamber and presents representative results of this wave on cultured lung epithelial cells.

Indexed as

bubblecell injuriesendothelial cellsepithelial cellsflowhemodynamicsparallel plate flow chamberstressviability

Identifiers

PMID32987681

DOI10.3390/mps3040065

PMCPMC7712398

OpenAlexW3088184894

What OpenQuestion holds

Textmetadata

LicenceCC BY

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.