Evidence map›Paper›PMID 32987208›Full record

ArticleInternational journal of surgery (London, England)2020

Does expression of a human complement-regulatory protein on xenograft cells protect them from systemic complement activation?

Abhijit Jagdale, Huy Nguyen, Juan Li, KaLia Burnette, David Ayares, David K C Cooper, Hidetaka Hara

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of surgery (London, England), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.8field-weighted citation impact, top 26% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 7 citations in OpenAlex.

  1. Article
  2. Article
  3. The Evolution of Immunosuppressive Therapy in Pig-to-Nonhuman Primate Organ Transplantation.Transplant international : official journal of the European Society for Organ Transplantation · 2024
    Review
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 2 countries.

Abhijit JagdaleXenotransplantation Program, Department of Surgery, University of Alabama at Birmingham, Birmingham, AL, USA.
Huy NguyenXenotransplantation Program, Department of Surgery, University of Alabama at Birmingham, Birmingham, AL, USA.
Juan LiXenotransplantation Program, Department of Surgery, University of Alabama at Birmingham, Birmingham, AL, USA; Second Affiliated Hospital, University of South China, Hengyang City, Hunan, China.
KaLia BurnetteXenotransplantation Program, Department of Surgery, University of Alabama at Birmingham, Birmingham, AL, USA.
David AyaresRevivicor, Blacksburg, VA, USA.
David K C CooperXenotransplantation Program, Department of Surgery, University of Alabama at Birmingham, Birmingham, AL, USA.
Hidetaka HaraXenotransplantation Program, Department of Surgery, University of Alabama at Birmingham, Birmingham, AL, USA. Electronic address: harahjp@icloud.com.
University of Alabama at Birmingham · US

Funding

Prolonging life-supported pig kidney and heart graft survival in baboons by suppressing inflammationU19AI090959 · NIAID · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI COOPER, DAVID KC · 2010 to 2024
$26.3M
NIAID NIH HHS U19 AI090959
6 · The paper itself

Abstract

backgroundThere are many causes of systemic complement activation, which may have detrimental effects on a pig xenograft. Transgenic expression of one or more human complement-regulatory proteins (hCRPs), e.g., hCD46, provides some protection to the xenograft, but it is not known whether it protects the xenograft from the effects of systemic complement activation. We used wild-type (WT) pig aortic endothelial cells (pAECs) to activate complement, and determined whether the expression of hCD46 on a1,3galactosyltransferase gene-knockout (GTKO) pAECs protected them from injury.

methodsCFSE-labeled and non-labeled pAECs from a WT, a GTKO, or a GTKO/hCD46 pig were separately incubated with heat-inactivated pooled human serum in vitro. Antibody pre-bonded CFSE-labeled and non-labeled pAECs were mixed, and then incubated with rabbit complement. The complement-dependent cytotoxicity was measured by flow cytometry.

resultsThere was significantly less lysis of GTKO/CD46 pAECs (6%) by 50% human serum compared to that of WT (91%, p<0.001) or GTKO (32%, p<0.01) pAECs. The lysis of GTKO pAECs was significantly increased when mixed with WT pAECs (p<0.05). In contrast, there was no significant change in cytotoxicity of GTKO/CD46 pAECs when mixed with WT pAECs.

conclusionsThe expression of hCD46 protected pAECs from systemic complement activation.

Indexed as

Complement ActivationAnimalsAnimals, Genetically ModifiedAortaCytotoxicity, ImmunologicEndothelial CellsHeterograftsHumansMembrane Cofactor ProteinSwineCD46 protein, humanMembrane Cofactor ProteinCD46ComplementComplement-regulatory proteinPigXenotransplantation

Identifiers

PMID32987208
PMCPMC7686296
OpenAlexW3087777866

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.