ArticlePloS one2020
Genomic characterization of a diazotrophic microbiota associated with maize aerial root mucilage.
Article in PloS one, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Microbial innovations for climate-resilient agriculture: mechanisms, applications, and emerging technologies.World journal of microbiology & biotechnology · 2026Review
- Lactobacilli biology, applications and host interactions.Nature reviews. Microbiology · 2026Review
- Plant microbiome engineering: from inoculation to genome editing.Frontiers in microbiology · 2026Review
- Bacterial Isolation from Natural Grassland on Nitrogen-Free Agar Yields Many Strains Without Nitrogenase.Microorganisms · 2025Article
- Nitrogen fixation rates and aerial root production among maize landraces.Frontiers in plant science · 2025Article
- Glucosinolate structural diversity shapes recruitment of a metabolic network of leaf-associated bacteria.Nature communications · 2024Article
- Genomic Insights intoInternational journal of molecular sciences · 2024Article
- Antimicrobial Resistance Genes in Respiratory Bacteria from Weaned Dairy Heifers.Pathogens (Basel, Switzerland) · 2024Article
- Probiotic model for studying rhizosphere interactions of root exudates and the functional microbiome.The ISME journal · 2024Article
- The Cultured Microbiome of Pollinated Maize Silks Shifts after Infection withPathogens (Basel, Switzerland) · 2023Article
- Microbiota-mediated nitrogen fixation and microhabitat homeostasis in aerial root-mucilage.Microbiome · 2023Article
- Comparison of the Peel-Associated Epiphytic Bacteria of Anthocyanin-Rich "Sun Black" and Wild-Type Tomatoes under Organic and Conventional Farming.Microorganisms · 2022Article
- The Impact of Non-Nodulating Diazotrophic Bacteria in Agriculture: Understanding the Molecular Mechanisms That Benefit Crops.International journal of molecular sciences · 2022Review
- Phylogeny of Nitrogenase Structural and Assembly Components Reveals New Insights into the Origin and Distribution of Nitrogen Fixation across Bacteria and Archaea.Microorganisms · 2021Article
- Genome-Based Characterization of Plant-AssociatedFrontiers in microbiology · 2021Article
- Identification of Nitrogen Fixation Genes inMicroorganisms · 2020Article
- Diazotrophic bacteria from maize exhibit multifaceted plant growth promotion traits in multiple hosts.PloS one · 2020Article
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9 authors.
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No grant is acknowledged in the PubMed record.
Abstract
A geographically isolated maize landrace cultivated on nitrogen-depleted fields without synthetic fertilizer in the Sierra Mixe region of Oaxaca, Mexico utilizes nitrogen derived from the atmosphere and develops an extensive network of mucilage-secreting aerial roots that harbors a diazotrophic (N2-fixing) microbiota. Targeting these diazotrophs, we selected nearly 600 microbes of a collection obtained from mucilage and confirmed their ability to incorporate heavy nitrogen (15N2) metabolites in vitro. Sequencing their genomes and conducting comparative bioinformatic analyses showed that these genomes had substantial phylogenetic diversity. We examined each diazotroph genome for the presence of nif genes essential to nitrogen fixation (nifHDKENB) and carbohydrate utilization genes relevant to the mucilage polysaccharide digestion. These analyses identified diazotrophs that possessed the canonical nif gene operons, as well as many other operon configurations with concomitant fixation and release of >700 different 15N labeled metabolites. We further demonstrated that many diazotrophs possessed alternative nif gene operons and confirmed their genomic potential to derive chemical energy from mucilage polysaccharide to fuel nitrogen fixation. These results confirm that some diazotrophic bacteria associated with Sierra Mixe maize were capable of incorporating atmospheric nitrogen into their small molecule extracellular metabolites through multiple nif gene configurations while others were able to fix nitrogen without the canonical (nifHDKENB) genes.
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