ArticlePLoS genetics2020
Cocoonase is indispensable for Lepidoptera insects breaking the sealed cocoon.
Article in PLoS genetics, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- CRISPR-Mediated Silkworm: The Oncoming Agricultural Revolutions and a Rising Model Organism.Genes · 2026Review
- Molecular, Structural and Functional Characterization of Antheraea mylitta Expressed Cocoonase: An Integrative Approach.The protein journal · 2025Article
- Telomere-to-telomere genome assemblies of three silkworm strains with long-term pupal characteristics.Scientific data · 2025Article
- Impression of Insect's Proteolytic Enzyme Cocoonase and Its Application: A Comprehensive Review.The protein journal · 2025Review
- Characterization and comparative analysis of sericin protein 150 in Bombyx mori.Scientific reports · 2024Article
- Transcriptome Analysis Provides Insights into Water Immersion Promoting the Decocooning ofInsects · 2024Article
- Silk fibroin-based scaffolds for tissue engineering.Frontiers in bioengineering and biotechnology · 2024Review
- Evolutionary dynamics of genome size and content during the adaptive radiation of Heliconiini butterflies.Nature communications · 2023Article
- Deciphering the Genetic Basis of Silkworm Cocoon Colors Provides New Insights into Biological Coloration and Phenotypic Diversification.Molecular biology and evolution · 2023Article
- In silico structural and functional characterization of Antheraea mylitta cocoonase.Journal, genetic engineering & biotechnology · 2022Article
- Dynamic monitoring of vital functions and tissue re-organization in Saturnia pavonia (Lepidoptera, Saturniidae) during final metamorphosis by non-invasive MRI.Scientific reports · 2022Article
- Expansion of targetable sites for the ribonucleoprotein-based CRISPR/Cas9 system in the silkworm Bombyx mori.BMC biotechnology · 2021Article
- Selection and evaluation of dual reference genes in silkwormScience progressArticle
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Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Many insects spin cocoons to protect the pupae from unfavorable environments and predators. After emerging from the pupa, the moths must escape from the sealed cocoons. Previous works identified cocoonase as the active enzyme loosening the cocoon to form an escape-hatch. Here, using bioinformatics tools, we show that cocoonase is specific to Lepidoptera and that it probably existed before the occurrence of lepidopteran insects spinning cocoons. Despite differences in cocooning behavior, we further show that cocoonase evolved by purification selection in Lepidoptera and that the selection is more intense in lepidopteran insects spinning sealed cocoons. Experimentally, we applied gene editing techniques to the silkworm Bombyx mori, which spins a dense and sealed cocoon, as a model of lepidopteran insects spinning sealed cocoons. We knocked out cocoonase using the CRISPR/Cas9 system. The adults of homozygous knock-out mutants were completely formed and viable but stayed trapped and died naturally in the cocoon. This is the first experimental and phenotypic evidence that cocoonase is the determining factor for breaking the cocoon. This work led to a novel silkworm strain yielding permanently intact cocoons and provides a new strategy for controlling the pests that form cocoons.
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