Evidence map›Paper›PMID 32985598›Full record

ArticleScientific reports2020

Expanded Chinese hamster organ and cell line proteomics profiling reveals tissue-specific functionalities.

Kelley Heffner, Deniz Baycin Hizal, Natalia I Majewska, Swetha Kumar, Venkata Gayatri Dhara, Jie Zhu, Michael Bowen, Diane Hatton, George Yerganian, Athena Yerganian and 3 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
1.0field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 18 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
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  5. Article
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  7. Review
  8. A proteomics approach to decipher a sticky CHO situation.Biotechnology and bioengineering · 2022
    Article
  9. Hyperosmolality in CHO cell culture: effects on the proteome.Applied microbiology and biotechnology · 2022
    Article
  10. Article
  11. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 3 institutions in 1 country.

Kelley HeffnerDepartment of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MD, USA.ORCID http://orcid.org/0000-0002-5459-2879
Deniz Baycin HizalDepartment of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MD, USA.
Natalia I MajewskaDepartment of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MD, USA.
Swetha KumarDepartment of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MD, USA.
Venkata Gayatri DharaDepartment of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MD, USA.
Jie ZhuAstraZeneca, Cell Culture and Fermentation Sciences, Gaithersburg, MD, USA.
Michael BowenAllogene Therapeutics, Product and Process Development, South San Francisco, CA, USA.
Diane HattonAstraZeneca, Cell Culture and Fermentation Sciences, Gaithersburg, MD, USA.
George YerganianCytogen Research and Development, Inc, Boston, MA, USA.
Athena YerganianCytogen Research and Development, Inc, Boston, MA, USA.
Robert O'MeallyDepartment of Pathology, Johns Hopkins School of Medicine, Baltimore, MD, USA.
Robert ColeDepartment of Pathology, Johns Hopkins School of Medicine, Baltimore, MD, USA.ORCID http://orcid.org/0000-0002-3096-4754
Michael BetenbaughDepartment of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MD, USA. beten@jhu.edu.
Johns Hopkins University · USAstraZeneca (United States) · USAllogene Therapeutics (United States) · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Chinese hamster ovary (CHO) cells are the predominant production vehicle for biotherapeutics. Quantitative proteomics data were obtained from two CHO cell lines (CHO-S and CHO DG44) and compared with seven Chinese hamster (Cricetulus griseus) tissues (brain, heart, kidney, liver, lung, ovary and spleen) by tandem mass tag (TMT) labeling followed by mass spectrometry, providing a comprehensive hamster tissue and cell line proteomics atlas. Of the 8470 unique proteins identified, high similarity was observed between CHO-S and CHO DG44 and included increases in proteins involved in DNA replication, cell cycle, RNA processing, and chromosome processing. Alternatively, gene ontology and pathway analysis in tissues indicated increased protein intensities related to important tissue functionalities. Proteins enriched in the brain included those involved in acidic amino acid metabolism, Golgi apparatus, and ion and phospholipid transport. The lung showed enrichment in proteins involved in BCAA catabolism, ROS metabolism, vesicle trafficking, and lipid synthesis while the ovary exhibited enrichments in extracellular matrix and adhesion proteins. The heart proteome included vasoconstriction, complement activation, and lipoprotein metabolism enrichments. These detailed comparisons of CHO cell lines and hamster tissues will enhance understanding of the relationship between proteins and tissue function and pinpoint potential pathways of biotechnological relevance for future cell engineering.

Indexed as

AnimalsBrainCell CycleCHO CellsChromosomes, MammalianCricetulusDNA ReplicationFemaleKidneyLungMyocardiumOvaryProteinsProteomicsSpleenTandem Mass SpectrometryProteins

Identifiers

PMID32985598
PMCPMC7522264
OpenAlexW3089143455

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.