Evidence map›Paper›PMID 32984852›Full record

ArticleSTAR protocols2020

Protocol for Purification of Human ZGRF1 and Its Regulatory Function on RAD51-Mediated D-Loop Formation.

Michael Lisby, Xiaoyu Xue

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in STAR protocols, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 92% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 2 institutions in 2 countries.

Michael LisbyDepartment of Biology, University of Copenhagen, 2200 Copenhagen N, Denmark.
Xiaoyu XueDepartment of Chemistry and Biochemistry, Texas State University, San Marcos, TX 78666, USA.
Texas State University · USUniversity of Copenhagen · DK

Funding

Roles of the nucleic acid motor protein ZGRF1 in chromosome damage repairR21ES029513 · NIEHS · YALE UNIVERSITY · PI SUNG, PATRICK · 2018 to 2019
$438k
Functions of the DNA/RNA motor protein AQR in R-loop resolutionR21ES028792 · NIEHS · YALE UNIVERSITY · PI XUE, XIAOYU · 2018 to 2019
$424k
NIEHS NIH HHS R21 ES028792NIEHS NIH HHS R21 ES029513
6 · The paper itself

Abstract

Human ZGRF1 is one of the many helicases that play important roles in the repair of damaged chromosomes by homologous recombination. However, the lack of biochemical characterization of the ZGRF1 protein has hindered the understanding of its function. We have developed a facile protocol to express human ZGRF1 in insect cells and for its purification. We have also tuned biochemical assays by lowering the protein and DNA concentrations to analyze its functions in RAD51-mediated D-loop formation and dissociation. For complete details on the use and execution of this protocol, please refer to Brannvoll et al. (2020).

Indexed as

DNADNA HelicasesHomologous RecombinationHumansMembrane ProteinsProteinsRad51 RecombinaseDNADNA HelicasesMembrane ProteinsProteinsRAD51 protein, humanRad51 RecombinaseZGRF1 protein, human

Identifiers

PMID32984852
PMCPMC7518512
OpenAlexW3082060250

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.