Evidence map›Paper›PMID 32981997›Full record

ArticlePlant cell, tissue and organ culture2018

Meta-topolin stimulates de novo shoot organogenesis and plant regeneration in cassava.

Raj Deepika Chauhan, Nigel James Taylor

Abstract read
In one paragraph

Article in Plant cell, tissue and organ culture, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Review
  5. Article
  6. Article
  7. Plant regeneration inHeliyon · 2020
    Article
  8. Protocol for Rescuing Young Cassava Embryos.Frontiers in plant science · 2020
    Article
  9. Article
  10. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Raj Deepika ChauhanDonald Danforth Plant Science Center, 975 North Warson Road, St. Louis, MO 63132, USA.ORCID 0000-0002-5426-2247
Nigel James TaylorDonald Danforth Plant Science Center, 975 North Warson Road, St. Louis, MO 63132, USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A novel protocol for de novo shoot organogenesis from cassava has been developed utilizing meta-topolin to stimulate shoot regeneration from leaf, petiole and stem internode explants. While use of meta-topolin alone was capable of inducing shoot regeneration, a two-stage system combining meta-topolin with 2,4-D in a first stage medium, followed by subculture onto elevated levels of meta-topolin, was superior for inducing multiple shoot regeneration events in more than 35% of explants in cultivar TME 7. Caulogenesis was achieved in eleven additional cultivars. Metatopolin was also found to be beneficial for stimulating shoot regeneration from somatic embryos and cotyledon explants. The shoot organogenesis techniques described enhance the capacity of existing embryogenic systems and present previously unavailable morphogenic pathways for developing genetic transformation and gene editing technologies in cassava.

Indexed as

CassavaMeta-topolinOrganogenesis Shoot regeneration

Identifiers

PMID32981997
PMCPMC7507842

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.