Evidence map›Paper›PMID 32980660›Full record

ArticleAutonomic neuroscience : basic & clinical2020

Effects of optogenetic activation of the enteric nervous system on gastrointestinal motility in mouse small intestine.

Nick J Spencer, Lee Travis, Tim Hibberd, Nigel Kelly, Jing Feng, Hongzhen Hu

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Autonomic neuroscience : basic & clinical, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.8field-weighted citation impact, top 32% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 10 citations in OpenAlex.

  1. Direct Stimulation of Gastric Smooth Muscle Cells via GNeurogastroenterology and motility · 2025
    Article
  2. The enteric nervous system.Physiological reviews · 2023
    Review
  3. Review
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 2 countries.

Nick J SpencerVisceral Neurophysiology Laboratory, College of Medicine and Public Health, Centre for Neuroscience, Flinders University, Bedford Park, South Australia 5042, Australia. Electronic address: nicholas.spencer@flinders.edu.au.
Lee TravisVisceral Neurophysiology Laboratory, College of Medicine and Public Health, Centre for Neuroscience, Flinders University, Bedford Park, South Australia 5042, Australia.
Tim HibberdVisceral Neurophysiology Laboratory, College of Medicine and Public Health, Centre for Neuroscience, Flinders University, Bedford Park, South Australia 5042, Australia.
Nigel KellySA Biomedical Engineering, SA Health, Government of South Australia, Australia.
Jing FengDepartment of Anesthesiology, The Center for the Study of Itch, Washington University, St Louis, MO, USA.
Hongzhen HuDepartment of Anesthesiology, The Center for the Study of Itch, Washington University, St Louis, MO, USA. Electronic address: hongzhen.hu@wustl.edu.
Flinders University · AUWashington University in St. Louis · USGovernment of South Australia · AU

Funding

Washington University DDRCC Supplemental Equipment RequestP30DK052574 · NIDDK · WASHINGTON UNIVERSITY · PI Nicholas O. Davidson · 2000 to 2026
$30.8M
TARGETING THE TRANSIENT RECEPTOR POTENTIAL CHANNELS TO IMPROVE BOWEL DYSFUNCTIONR01DK103901 · NIDDK · WASHINGTON UNIVERSITY · PI HU, HONGZHEN · 2015 to 2023
$3.4M
Mechanisms of Zinc Regulation of Pain-initiating TRP ChannelsR01GM101218 · NIGMS · WASHINGTON UNIVERSITY · PI HU, HONGZHEN · 2013 to 2017
$1.4M
NIDDK NIH HHS P30 DK052574NIDDK NIH HHS R01 DK103901NIGMS NIH HHS R01 GM101218
6 · The paper itself

Abstract

BACKGROUND AND

aimsRecently, it was demonstrated that optogenetics could be used to stimulate enteric calretinin neurons, leading to increased colonic transit in vitro and in vivo. The aim of the current study was to determine if similar approaches could be used to stimulate the isolated mouse small intestine, with the aim of potentially also improving transit in the small bowel.

methodsCre-Lox recombination was used to generate transgenic mice expressing the light-sensitive ion channel channelrhodopsin-2 (ChR2) in calretinin neurons.

resultsSpontaneous migrating motor complexes were recorded from isolated terminal small intestine in both Cal

conclusionFocal illumination of the small intestine does not appear as effective at inducing propulsive motor activity as has been demonstrated in the colon of the same colony mice. This study suggests caution should be exercised when assuming optogenetic technology is equally effective at increasing GI transit in the small intestine as in the large intestine of mice.

Indexed as

AnimalsCalbindin 2ChannelrhodopsinsEnteric Nervous SystemGastrointestinal MotilityImmunohistochemistryIntestine, SmallMiceMice, TransgenicOptogeneticsSensory Receptor CellsCalb2 protein, mouseCalbindin 2ChannelrhodopsinsAutonomic nervous systemColonEnteric nervous systemMotilityPeristalsisSensory neuron

Identifiers

PMID32980660
PMCPMC9884517
OpenAlexW3087710819

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.