ArticleEBioMedicine2020
Hypothermic oxygenated perfusion protects from mitochondrial injury before liver transplantation.
Article in EBioMedicine, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 115 papers, 4 of them syntheses that pooled it.
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Who cites it
115 citing papers in PubMed, 4 syntheses or guidelines pooled it.
- The role of machine perfusion in preventing recurrence after liver transplant for hepatocellular carcinoma: a systematic review and meta-analysis.BMC gastroenterology · 2026Pooled it
- Hypothermic Oxygenated Perfusion Versus Static Cold Storage in Transplantation of Extended Criteria Liver Grafts: A Systematic Review and Meta-Analysis.Clinical transplantation · 2025Pooled it
- Association between Hepatocellular Carcinoma Recurrence and Graft Size in Living Donor Liver Transplantation: A Systematic Review.International journal of molecular sciences · 2023Pooled it
- Mitochondrial Consequences of Organ Preservation Techniques during Liver Transplantation.International journal of molecular sciences · 2021Pooled it
- Mitochondrial respiration during normothermic liver machine perfusion predicts clinical outcome.EBioMedicine · 2022Trial
- The Association of Flavin Mononucleotide and Lactate During Normothermic Regional Perfusion with Post-Transplant Liver Outcomes: An Exploratory Study.Medicina (Kaunas, Lithuania) · 2026Article
- Suitability of a Fluorescence Method for Flavin Mononucleotide Determination in Modified HTK-Based Perfusion Solutions During Hypothermic Oxygenated Perfusion of Liver Grafts.Methods and protocols · 2026Article
- Indocyanine green fluorescence kinetics during hypothermic oxygenated machine perfusion might predict graft function after liver transplantation.Langenbeck's archives of surgery · 2026Article
- Review
- From Rescue to Risk: Challenges and Controversies in Pushing the Boundaries of Organ Utilization by Machine Perfusion.Artificial organs · 2026Review
- Hydrogen gas with hypothermic machine perfusion induces lipidomic alterations in donation-after-cardiac-death rat livers.Journal of lipid research · 2026Article
- Optimal coronary flow rates for preservation of function during normothermic ex situ heart perfusion.The Journal of thoracic and cardiovascular surgery · 2026Article
- Protective effect of Caftaric Acid against hepatic cold ischemia/reperfusion injury in the liver.Biology direct · 2026Article
- Single-cell transcriptomic profiling reveals cellular stress responses to hypothermic preservation in liver-on-chip model.Scientific reports · 2026Article
- Fluorescence spectroscopy precorrection model robust to large variability of optical properties: application to point of care quantitative liver graft viability assessment.Biomedical optics express · 2026Article
- First Experience with Hypothermic Oxygenated Perfusion in Human Uteri: Feasibility and Metabolic Characterization.Journal of clinical medicine · 2026Article
- Prolonged Dual Hypothermic Oxygenated Machine Perfusion for Daytime Liver Transplant.JAMA network open · 2026Article
- NGR1 Ameliorates Hepatocyte Steatosis and Mitochondrial Dysfunction Associated with the Restoration of NDUFS2.Pharmaceuticals (Basel, Switzerland) · 2026Article
- Small animalWorld journal of gastroenterology · 2026Review
- HCC recurrence in liver transplants treated with hypothermic oxygenated machine perfusion: An international matched cohort study.JHEP reports : innovation in hepatology · 2026Article
55 more citing papers are in PubMed but not listed here.
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Authors and funding
11 authors.
Funding
Abstract
backgroundMitochondrial succinate accumulation has been suggested as key event for ischemia reperfusion injury in mice. No specific data are however available on behavior of liver mitochondria during ex situ machine perfusion in clinical transplant models.
methodsWe investigated mitochondrial metabolism of isolated perfused rat livers before transplantation. Livers were exposed to warm and cold ischemia to simulate donation after circulatory death (DCD) and organ transport. Subsequently, livers were perfused with oxygenated Belzer-MPS for 1h, at hypothermic or normothermic conditions. Various experiments were performed with supplemented succinate and/or mitochondrial inhibitors. The perfusate, liver tissues, and isolated mitochondria were analyzed by mass-spectroscopy and fluorimetry. Additionally, rat DCD livers were transplanted after 1h hypothermic or normothermic oxygenated perfusion. In parallel, perfusate samples were analysed during HOPE-treatment of human DCD livers before transplantation.
findingsSuccinate exposure during rat liver perfusion triggered a dose-dependent release of mitochondrial Flavin-Mononucleotide (FMN) and NADH in perfusates under normothermic conditions. In contrast, perfusate FMN was 3-8 fold lower under hypothermic conditions, suggesting less mitochondrial injury during cold re-oxygenation compared to normothermic conditions. HOPE-treatment induced a mitochondrial reprogramming with uploading of the nucleotide pool and effective succinate metabolism. This resulted in a clear superiority after liver transplantation compared to normothermic perfusion. Finally, the degree of mitochondrial injury during HOPE of human DCD livers, quantified by perfusate FMN and NADH, was predictive for liver function.
interpretationMitochondrial injury determines outcome of transplanted rodent and human livers. Hypothermic oxygenated perfusion improves mitochondrial function, and allows viability assessment of liver grafts before implantation.
fundingdetailed information can be found in Acknowledgments.
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