Evidence map›Paper›PMID 32979514›Full record

ArticleFungal genetics and biology : FG & B2021

Live-cell imaging of rapid calcium dynamics using fluorescent, genetically-encoded GCaMP probes with Aspergillus fumigatus.

Alberto Muñoz, Margherita Bertuzzi, Constanze Seidel, Darren Thomson, Elaine M Bignell, Nick D Read

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Fungal genetics and biology : FG & B, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.6field-weighted citation impact, top 34% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 9 citations in OpenAlex.

  1. Article
  2. Harnessing Fungi Signaling in Living Composites.Global challenges (Hoboken, NJ) · 2024
    Article
  3. Article
  4. Article
  5. Article
  6. Aequorin as a Useful Calcium-Sensing Reporter inJournal of fungi (Basel, Switzerland) · 2021
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Alberto MuñozManchester Fungal Infection Group, Division of Infection, Immunity and Respiratory Medicine, University of Manchester, CTF Building, 46 Grafton Street, Manchester M13 9NT, UK.
Margherita BertuzziManchester Fungal Infection Group, Division of Infection, Immunity and Respiratory Medicine, University of Manchester, CTF Building, 46 Grafton Street, Manchester M13 9NT, UK.
Constanze SeidelManchester Fungal Infection Group, Division of Infection, Immunity and Respiratory Medicine, University of Manchester, CTF Building, 46 Grafton Street, Manchester M13 9NT, UK.
Darren ThomsonManchester Fungal Infection Group, Division of Infection, Immunity and Respiratory Medicine, University of Manchester, CTF Building, 46 Grafton Street, Manchester M13 9NT, UK.
Elaine M BignellManchester Fungal Infection Group, Division of Infection, Immunity and Respiratory Medicine, University of Manchester, CTF Building, 46 Grafton Street, Manchester M13 9NT, UK. Electronic address: E.Bignell@exeter.ac.uk.
Nick D ReadManchester Fungal Infection Group, Division of Infection, Immunity and Respiratory Medicine, University of Manchester, CTF Building, 46 Grafton Street, Manchester M13 9NT, UK.
University of Manchester · GB

Funding

Medical Research Council G0501164Medical Research Council MR/L000822/1Medical Research Council MR/N006364/2Medical Research Council MR/S001824/1Medical Research Council MR/S001824/2Wellcome Trust 093596Wellcome Trust WT093596/A/10/ZWellcome Trust WT093596/C/10/Z
6 · The paper itself

Abstract

Calcium signalling plays a fundamental role in fungal intracellular signalling. Previous approaches (fluorescent dyes, bioluminescent aequorin, genetically encoded cameleon probes) with imaging rapid subcellular changes in cytosolic free calcium ([Ca

Indexed as

Genes, ReporterAspergillus fumigatusCalciumCalcium SignalingCalmodulinFluorescent DyesGreen Fluorescent ProteinsMolecular ProbesMyosin-Light-Chain KinaseSpores, FungalCalciumCalmodulinFluorescent DyesGreen Fluorescent ProteinsMolecular ProbesMyosin-Light-Chain KinaseAspergillus fumigatusCalcium imagingGCaMP calcium probesLive-cell imaging

Identifiers

PMID32979514
PMCPMC7617832
OpenAlexW3088804074

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.