Evidence map›Paper›PMID 32979500›Full record

ArticleArthroscopy : the journal of arthroscopic & related surgery : official publication of the Arthroscopy Association of North America and the International Arthroscopy Association2021

Assessing the Resident Progenitor Cell Population and the Vascularity of the Adult Human Meniscus.

Jorge Chahla, Angela Papalamprou, Virginia Chan, Yasaman Arabi, Khosrawdad Salehi, Trevor J Nelson, Orr Limpisvasti, Bert R Mandelbaum, Wafa Tawackoli, Melodie F Metzger and 1 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Arthroscopy : the journal of arthroscopic & related surgery : official publication of the Arthroscopy Association of North America and the International Arthroscopy Association, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
31citing papers in PubMed, 1 pooled it
5.5field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

31 citing papers in PubMed, 1 synthesis or guideline pooled it, 46 citations in OpenAlex.

  1. Meniscal repair: The current state and recent advances in augmentation.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2021
    Pooled it
  2. Hybrid Sequential Meniscal Suture Technique.Arthroscopy techniques · 2026
    Article
  3. Review
  4. Article
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  8. Review
  9. Article
  10. The clinical potential of meniscal progenitor cells.The journal of cartilage & joint preservation · 2024
    Review
  11. Review
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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

11 authors at 2 institutions in 2 countries.

Jorge ChahlaKerlan Jobe Institute, Cedars-Sinai Medical Center, Los Angeles, California, U.S.A.
Angela PapalamprouOrthopedic Stem Cell Research Laboratory, Cedars-Sinai Medical Center, Los Angeles, California, U.S.A.; Board of Governors Regenerative Medicine Institute, Cedars-Sinai Medical Center, Los Angeles, California, U.S.A.
Virginia ChanOrthopedic Stem Cell Research Laboratory, Cedars-Sinai Medical Center, Los Angeles, California, U.S.A.; Board of Governors Regenerative Medicine Institute, Cedars-Sinai Medical Center, Los Angeles, California, U.S.A.
Yasaman ArabiOrthopedic Stem Cell Research Laboratory, Cedars-Sinai Medical Center, Los Angeles, California, U.S.A.; Board of Governors Regenerative Medicine Institute, Cedars-Sinai Medical Center, Los Angeles, California, U.S.A.
Khosrawdad SalehiOrthopedic Stem Cell Research Laboratory, Cedars-Sinai Medical Center, Los Angeles, California, U.S.A.; Board of Governors Regenerative Medicine Institute, Cedars-Sinai Medical Center, Los Angeles, California, U.S.A.
Trevor J NelsonDepartment of Orthopedics, Cedars-Sinai Medical Center, Los Angeles, California, U.S.A.
Orr LimpisvastiKerlan Jobe Institute, Cedars-Sinai Medical Center, Los Angeles, California, U.S.A.
Bert R MandelbaumKerlan Jobe Institute, Cedars-Sinai Medical Center, Los Angeles, California, U.S.A.
Wafa TawackoliOrthopedic Stem Cell Research Laboratory, Cedars-Sinai Medical Center, Los Angeles, California, U.S.A.; Board of Governors Regenerative Medicine Institute, Cedars-Sinai Medical Center, Los Angeles, California, U.S.A.; Department of Surgery, Cedars-Sinai Medical Center, Los Angeles, California, U.S.A.; Department of Biomedical Sciences, Cedars-Sinai Medical Center, Los Angeles, California, U.S.A.
Melodie F MetzgerDepartment of Orthopedics, Cedars-Sinai Medical Center, Los Angeles, California, U.S.A.
Dmitriy SheynKerlan Jobe Institute, Cedars-Sinai Medical Center, Los Angeles, California, U.S.A.; Orthopedic Stem Cell Research Laboratory, Cedars-Sinai Medical Center, Los Angeles, California, U.S.A.; Department of Orthopedics, Cedars-Sinai Medical Center, Los Angeles, California, U.S.A.; Department of Surgery, Cedars-Sinai Medical Center, Los Angeles, California, U.S.A.; Department of Biomedical Sciences, Cedars-Sinai Medical Center, Los Angeles, California, U.S.A.. Electronic address: dmitry.sheyn@csmc.edu.
Cedars-Sinai Medical Center · USCedars-Sinai Kerlan-Jobe Institute · US

Funding

Induced pluripotent stem cell therapy for degenerated IVDK01AR071512 · NIAMS · CEDARS-SINAI MEDICAL CENTER · PI SHEYN, DMITRIY · 2018 to 2022
$595k
NIAMS NIH HHS K01 AR071512
6 · The paper itself

Abstract

purposeTo identify, characterize, and compare the resident progenitor cell populations within the red-red, red-white, and white-white (WW) zones of freshly harvested human cadaver menisci and to characterize the vascularity of human menisci using immunofluorescence and 3-dimensional (3D) imaging.

methodsFresh adult human menisci were harvested from healthy donors. Menisci were enzymatically digested, mononuclear cells isolated, and characterized using flow cytometry with antibodies against mesenchymal stem cell surface markers (CD105, CD90, CD44, and CD29). Cells were expanded in culture, characterized, and compared with bone marrow-derived mesenchymal stem cells. Trilineage differentiation potential of cultured cells was determined. Vasculature of menisci was mapped in 3D using a modified uDisco clearing and immunofluorescence against vascular markers CD31, lectin, and alpha smooth muscle actin.

resultsThere were no significant differences in the clonogenicity of isolated cells between the 3 zones. Flow cytometry showed presence of CD44

conclusionsThe presence of resident mesenchymal progenitors was evident in all 3 meniscal zones of healthy adult donors without injury. In addition, our results demonstrate the presence of vascularization in the WW zone. CLINICAL RELEVANCE: The existence of progenitors and presence of microvasculature in the WW zone of the meniscus suggests the potential for repair and biologic augmentation strategies in that zone of the meniscus in young healthy adults. Further research is necessary to fully define the functionality of the meniscal blood supply and its implications for repair.

Indexed as

CadaverCell DifferentiationCells, CulturedFlow CytometryHumansMeniscusMesenchymal Stem CellsStem CellsYoung Adult

Identifiers

PMID32979500
PMCPMC7829352
OpenAlexW3087874567

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.