Evidence map›Paper›PMID 32979302›Full record

ArticleVeterinary medicine and science2021

A rapid and simple single-step method for the purification of Toxoplasma gondii tachyzoites and bradyzoites.

Saeed El-Ashram, Yu Zhang, Yongsheng Ji, Dina Salama, Kun Mei, Li Zhili, Huang Shujian, Haoji Zhang, Shawky M Aboelhadid, Reem A Alajmi and 12 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Veterinary medicine and science, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 53% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

22 authors at 14 institutions in 7 countries.

Saeed El-AshramCollege of Life Science and Engineering, Foshan University, Foshan, Guangdong Province, China.ORCID 0000-0003-0389-1980
Yu ZhangCollege of Life Science and Engineering, Foshan University, Foshan, Guangdong Province, China.
Yongsheng JiSchool of Basic Medical Sciences, Division of Life Science and Medicine, University of Science and Technology of China, Hefei, China.
Dina SalamaDepartment of Parasitology and Animal Disease, National Research Centre, Dokki, Giza, Egypt.
Kun MeiCollege of Life Science and Engineering, Foshan University, Foshan, Guangdong Province, China.
Li ZhiliCollege of Life Science and Engineering, Foshan University, Foshan, Guangdong Province, China.
Huang ShujianCollege of Life Science and Engineering, Foshan University, Foshan, Guangdong Province, China.
Haoji ZhangCollege of Life Science and Engineering, Foshan University, Foshan, Guangdong Province, China.
Shawky M AboelhadidParasitology Department, Faculty of Veterinary Medicine, Beni Suef University, Beni-Suef, Egypt.
Reem A AlajmiDepartment of Zoology, Faculty of Science, King Saud University, Riyadh, Saudi Arabia.
Dina M MetwallyDepartment of Zoology, Faculty of Science, King Saud University, Riyadh, Saudi Arabia.
Manal F El-KhadragyDepartment of Zoology and Entomology, Faculty of Science, Helwan University, Cairo, Egypt.
Billy M HargisDepartment of Poultry Science, University of Arkansas, Fayetteville, AR, USA.
Guillermo Tellez-IsaiasDepartment of Poultry Science, University of Arkansas, Fayetteville, AR, USA.
Beniamino T Cenci-GogaDipartimento di Medicina Veterinaria, Laboratorio di Ispezione degli Alimenti di Origine Animale, University of Perugia, Perugia, Italy.
Musafiri KaramaDepartment of Paraclinical Sciences, Faculty of Veterinary Science, University of Pretoria, Onderstepoort, South Africa.
Munyaradzi C MarufuDepartment of Veterinary Tropical Diseases, Faculty of Veterinary Science, University of Pretoria, Onderstepoort, South Africa.
Fathi AbouhajerFaculty of Education, Asmarya University for Islamic Sciences, Zliten, Libya.
Gamal Ali Abdelhafez HamadyDepartment of Animal Production, Faculty of Agriculture, Al-Azhar University, Nasr City, Cairo, Egypt.
Abeer El WakilDepartment of Biological & Geological Sciences, Alexandria University, Alexandria, Egypt.
Ibrahim Al NasrCollege of Science and Arts in Unaizah, Qassim University, Unaizah, Saudi Arabia.
Xun SuoNational Animal Protozoa Laboratory & College of Veterinary Medicine, China Agricultural University, Beijing, China.
Foshan University · CNBeni-Suef University · EGKing Saud University · SAOnderstepoort Veterinary Academic Hospital · ZAUniversity of Arkansas at Fayetteville · USAlasmarya Islamic University · LYAl-Azhar University · EGAlexandria University · EGChina Agricultural University · CNHelwan University · EGKafrelsheikh University · EGQassim University · SAUniversity of Perugia · ITUniversity of Science and Technology of China · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This study describes a simple method for the large-scale isolation of pure Toxoplasma gondii tachyzoites and bradyzoites. T. gondii tachyzoites were obtained from infected human foreskin fibroblasts (HFFs) and peritoneal exudates of mice, while tissue cysts containing bradyzoites were collected from chronically infected mice. Harvested cells and brain tissues were incubated in Hanks balanced salt solution (HBSS), containing 0.25% trypsin and 0.5% taurodeoxycholic acid (TDC) for 5 min. Subsequent washes in phosphate buffered saline (PBS) were conducted, and the cell viability of the preparations was good, as determined by flow cytometry and ability to reinfect HFF cells and propagate in mice. The purification procedure allowed for a rapid preparation of pure T. gondii tachyzoites and bradyzoites in sufficient quantity that can be used for downstream procedures. The advantage of the new method is that it is convenient and inexpensive.

Indexed as

AnimalsHumansMiceParasitologyToxoplasmaVeterinary Medicineflow cytometrypurificationtaurodeoxycholic acidT. gondii tachyzoites and bradyzoitestrypsin

Identifiers

PMID32979302
PMCPMC8025613
OpenAlexW3089177748

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.