Evidence map›Paper›PMID 32979185›Full record

ArticleMolecular biotechnology2020

Identification of ACTB Gene as a Potential Safe Harbor Locus in Pig Genome.

Youcai Xiong, Xiaosong Han, Jinfu Zhang, Guangxing Zhao, Zichang Wang, Rongzhi Zhuang, Xiongwei Nie, Shengsong Xie, Changchun Li, Xinyun Li and 4 more

Abstract read
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In one paragraph

Article in Molecular biotechnology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.3field-weighted citation impact, top 42% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 7 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 3 institutions in 1 country.

Youcai XiongKey Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Huazhong Agricultural University, Ministry of Education, Wuhan, 430070, China.
Xiaosong HanKey Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Huazhong Agricultural University, Ministry of Education, Wuhan, 430070, China.
Jinfu ZhangKey Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Huazhong Agricultural University, Ministry of Education, Wuhan, 430070, China.
Guangxing ZhaoKey Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Huazhong Agricultural University, Ministry of Education, Wuhan, 430070, China.
Zichang WangKey Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Huazhong Agricultural University, Ministry of Education, Wuhan, 430070, China.
Rongzhi ZhuangKey Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Huazhong Agricultural University, Ministry of Education, Wuhan, 430070, China.
Xiongwei NieKey Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Huazhong Agricultural University, Ministry of Education, Wuhan, 430070, China.
Shengsong XieKey Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Huazhong Agricultural University, Ministry of Education, Wuhan, 430070, China.
Changchun LiKey Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Huazhong Agricultural University, Ministry of Education, Wuhan, 430070, China.
Xinyun LiKey Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Huazhong Agricultural University, Ministry of Education, Wuhan, 430070, China.
Xiangdong LiuKey Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Huazhong Agricultural University, Ministry of Education, Wuhan, 430070, China.
Kui LiState Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
Shuhong ZhaoKey Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Huazhong Agricultural University, Ministry of Education, Wuhan, 430070, China.
Jinxue RuanKey Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Huazhong Agricultural University, Ministry of Education, Wuhan, 430070, China. ruanjinxue@mail.hzau.edu.cn.ORCID http://orcid.org/0000-0002-0058-6924
Huazhong Agricultural University · CNChinese Academy of Agricultural Sciences · CNFoshan University · CN

Funding

Breeding of new varieties of genetically modified organisms Project 2018ZX0801010B-003Fundamental Research Funds for Central Universities of the Central South University 2662018QD002National Natural Science Foundation of China 31702078
6 · The paper itself

Abstract

Transgenic pigs play an important role in biomedicine and agriculture. The "safe harbor" locus maintains consistent foreign gene expression in cells and is important for transgenic pig generation. However, as only several safe harbor loci(Rosa26, pH11 and Pifs501) have been identified in pigs, meeting the needs of the insertion of various foreign genes is difficult. In this study, we develop a novel strategy for the efficient knock-in of gene-of-interest fragments into endogenous beta-actin(ACTB) gene via CRISPR/Cas9 mediated homologous recombination with normal expression of ACTB. Thus, we provide an alternative strategy to integrate exogenous genes into the pig genome that can be applied to agricultural breeding and biomedical models.

Indexed as

ActinsAnimalsAnimals, Genetically ModifiedCell LineCRISPR-Cas SystemsGene ExpressionGene Knock-In TechniquesGene TargetingGreen Fluorescent ProteinsHomologous RecombinationSwineActinsGreen Fluorescent ProteinsACTBCRISPR/Cas9Homologous recombinationPigSafe harbor

Identifiers

PMID32979185
OpenAlexW3088575657

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.