Knocking out Analysis of the CpxP gene using Crispr/Cas9 in Escherichia coli MG1655.

Xiaoliang He, Yuwen Ren, Wanli Meng, Xinran Yu, Xiaohui Zhou

Open access · goldAbstract read

In one paragraph

Article in AMB Express, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

0.3field-weighted citation impact, top 45% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 4 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Xiaoliang HeSchool of Biological Science and Engineering, Hebei University of Science and Technology, No. 26 Yuxiang Street, Shijiazhuang, 050018, Hebei, China.ORCID http://orcid.org/0000-0003-1671-166X

Yuwen RenSchool of Biological Science and Engineering, Hebei University of Science and Technology, No. 26 Yuxiang Street, Shijiazhuang, 050018, Hebei, China.

Wanli MengSchool of Biological Science and Engineering, Hebei University of Science and Technology, No. 26 Yuxiang Street, Shijiazhuang, 050018, Hebei, China.

Xinran YuSchool of Biological Science and Engineering, Hebei University of Science and Technology, No. 26 Yuxiang Street, Shijiazhuang, 050018, Hebei, China.

Xiaohui ZhouSchool of Biological Science and Engineering, Hebei University of Science and Technology, No. 26 Yuxiang Street, Shijiazhuang, 050018, Hebei, China. zhouxh2003@aliyun.com.

Hebei University of Science and Technology · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Based on the analysis of cpxP genes among Escherichia coli strains, cpxP gene-targeting short guide RNA (sgRNA) was designed and inserted into the pGL3-MGP-RNA. The donor sequences (MG-HR) for homologous repair were designed and cloned by PCR. MG-HR and pGL3-MGP-RNA were transformed into E. coli MG1655 (pCas9). The cpxP gene expression cassette was amplified by PCR and subcloned into pBBR1MCS-2. Then the pBBR-cpxP was independently transformed into E. coli MG1655. The results of motility experiment suggest that cpxP gene had a significant effect on the movement ability of E. coli strain. The CpxP protein had a significant inhibition of bacterial activity. The lastest 81 CpxP proteins sequences were selected and analyzed by multi-sequence alignment and molecular cluster. The CpxP proteins were roughly divided into three categories. Our results suggest that the CpxP protein was involved in bacterial motility, infection and pathogenicity.

Indexed as

cpxPCrispr/Cas9Escherichia coliHomologous repairKnocked out

Identifiers

PMID32979149

DOI10.1186/s13568-020-01099-z

PMCPMC7519928

OpenAlexW3088784997

What OpenQuestion holds

Textmetadata

LicenceCC BY

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.