ArticleMarine biotechnology (New York, N.Y.)2021
A Green Light-Regulated T7 RNA Polymerase Gene Expression System for Cyanobacteria.
Article in Marine biotechnology (New York, N.Y.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- Superfolder Green Fluorescent Protein (sfGFP): A Versatile Tool for Biotechnology Research.Applied biochemistry and biotechnology · 2026Review
- Innovations, Challenges and Future Directions of T7RNA Polymerase in Microbial Cell Factories.ACS synthetic biology · 2025Review
- A Golden Gate compatible system for continuous directed evolution inSynthetic biology (Oxford, England) · 2025Article
- Current Metabolic Engineering Strategies for Photosynthetic Bioproduction in Cyanobacteria.Microorganisms · 2023Review
- The Molecular Toolset and Techniques Required to Build Cyanobacterial Cell Factories.Advances in biochemical engineering/biotechnology · 2023Article
- Light-induced production of isobutanol and 3-methyl-1-butanol by metabolically engineered cyanobacteria.Microbial cell factories · 2022Article
- Approaches in the photosynthetic production of sustainable fuels by cyanobacteria using tools of synthetic biology.World journal of microbiology & biotechnology · 2021Review
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Authors and funding
8 authors.
Funding
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Abstract
In this study, we developed a green light-regulated T7 RNA polymerase expression system (T7 RNAP system), to provide a novel and versatile high-expression system for cyanobacteria without using any chemical inducer, realizing high expression levels comparable with previously reported for recombinant gene expression in cyanobacteria. The T7 RNAP system was constructed and introduced into Synechocystis sp. PCC6803. T7 RNAP was inserted downstream of the cpcG2 promoter, which is recognized and activated by the CcaS/CcaR two-component green-light-sensing system, to compose a vector plasmid, pKT-CS01, to achieve the induction of T7 RNAP expression only under green light illumination, with repression under red light illumination. The reporter gene, superfolder green fluorescent protein (sfGFP), was inserted downstream of the T7 promoter. Transcriptional analyses revealed that T7 RNAP was induced under green light but repressed under red light. Expression of the sfGFP protein derived from pKT-CS01 was observed under green light illumination and was approximately 10-fold higher than that in the control transformant, which expressed sfGFP directly under the cpcG2 promoter, which is directly regulated by CcaS/CcaR, under green light illumination. Comparison with the strong promoter expression systems P
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