ArticleCytometry. Part A : the journal of the International Society for Analytical Cytology2020
OMIP-070: NKp46-Based 27-Color Phenotyping to Define Natural Killer Cells Isolated From Human Tumor Tissues.
Article in Cytometry. Part A : the journal of the International Society for Analytical Cytology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 13 citations in OpenAlex.
- An at-home blood collection device for remote immune monitoring by high-parameter flow cytometry.JCI insight · 2026Article
- Phenotypically similar but functionally distinct NK cell populations within the human maternal-fetal interface.ImmunoHorizons · 2026Article
- Comprehensive immune profiling of human peripheral blood mononuclear cells using two complementary spectral flow cytometry panels encompassing 60 unique markers.Frontiers in immunology · 2026Article
- Mucosal tissue NK cells tune their function between optimal anti-pathogen activity and tissue protection.bioRxiv : the preprint server for biology · 2025Article
- A 41-marker 37-color full spectrum flow cytometry panel for the deep immunophenotyping of human peripheral and liver natural killer cells.Frontiers in immunology · 2025Article
- OMIP-102: 50-color phenotyping of the human immune system with in-depth assessment of T cells and dendritic cells.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2024Article
- Signals that control MAIT cell function in healthy and inflamed human tissues.Immunological reviews · 2024Review
- A novel NKp80-based strategy for universal identification of normal, reactive and tumor/clonal natural killer-cells in blood.Frontiers in immunology · 2024Article
- 50-color phenotyping of the human immune system with in-depth assessment of T cells and dendritic cells.bioRxiv : the preprint server for biology · 2023Article
- Advances and challenges in studying the tissue-resident T cell compartment in the human female reproductive tract.Immunological reviews · 2023Review
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
This 27-color panel has been validated and optimized to comprehensively profile natural killer (NK) cells isolated from human tumors using a collagenase Type II-based digestion protocol. We confirmed that detection of protein expression by antibodies used in our final panel was not affected during tissue digestion. During this evaluation process, we found that detection of CD56, a biomarker typically used to identify NK cells, was affected substantially by collagenase-based digestion. Thus, our panel is centered around expression of NKp46, which is sufficient to identify NK cells and not affected by the tissue collagenase digestion step. Our panel further includes biomarkers used to extrapolate NK-cell maturation, differentiation, migration, homing potential, and functional state. Our panel is intended to provide in-depth characterization of human NK cells isolated from tissues, which we specifically tested using oral squamous cell carcinomas tissues, but it is compatible with other tissues that can be dissociated with a collagenase Type II-based protocol. © 2020 The Authors. Cytometry Part A published by Wiley Periodicals LLC on behalf of International Society for Advancement of Cytometry.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.