ArticleScientific reports2020
Extracellular vesicles shuttle protective messages against heat stress in bovine granulosa cells.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers, 1 of them a synthesis that pooled it.
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Who cites it
41 citing papers in PubMed, 1 synthesis or guideline pooled it, 58 citations in OpenAlex.
- Meta-analysis of extracellular vesicles-associated protein abundance and aggregation during aging and disease in C. elegans.Biogerontology · 2025Pooled it
- Extracellular vesicles participate in proteostasis and heat shock adaptation inEmerging microbes & infections · 2026Article
- Extracellular Vesicles From Heat- and Oxidative-Stressed Granulosa Cells Improve Redox Balance and Embryo Development in Bovine Oocytes.Reproduction in domestic animals = Zuchthygiene · 2026Article
- Stress-Induced Switch in Small Extracellular Vesicle Secretion: From Constitutive 'Torn Bag Mechanism' to Exocytosis.Journal of extracellular vesicles · 2026Article
- Environmental Regulation, Molecular Profiling, and Preliminary Functional Evaluation of Extracellular Vesicles fromFoods (Basel, Switzerland) · 2026Article
- Mechanistic understanding of heat stress in cattle reproduction toward pharmacological strategies: review.Animal bioscience · 2026Article
- Changes in the microRNA cargo of granulosa cell-derived extracellular vesicles under oxidative stress in a bovine model.Biological research · 2026Article
- Characterization of bovine oviductal organoids: Polarity, cryopreservation, hormonal stimulation, and extracellular vesicles.Cell and tissue research · 2026Article
- The Influence of Ovarian-Derived Extracellular Vesicles in Reproduction.Advances in anatomy, embryology, and cell biology · 2026Review
- Integrated molecular analysis of granulosa cells and follicular fluid extracellular vesicles reveals predictive biomarkers of oocyte maturation in the southern white rhinoceros.Frontiers in cell and developmental biology · 2026Article
- Molecular and developmental consequences of heat stress on the bovine oocyte and embryo competence.Animal reproduction · 2026Article
- Six things to know about how heat stress compromises fertility of the cow (and probably other mammals as well).The Journal of reproduction and development · 2026Review
- Extracellular vesicles in monitoring and modulation of oocyte competence: focus on exosomes.Journal of ovarian research · 2025Review
- Bridging biology and therapy: translational advances of extracellular vesicles in veterinary clinical practice.Veterinary research communications · 2025Review
- Integrated Metabolomic and Transcriptomic Analysis Decodes Heat Stress-Induced Metabolic Shifts in Gilt Granulosa Cells.Veterinary sciences · 2025Article
- Effect of heat stress on some serum and reproductive parameters of Holstein dairy cows in Egypt: potential biomarkers.BMC veterinary research · 2025Article
- Enhancing developmental potential of vitrified in vitro matured bovine oocytes using extracellular vesicles from large follicles.Scientific reports · 2025Article
- Integrating Near-Infrared Spectroscopy and Proteomics for Semen Quality Biosensing.Biosensors · 2025Article
- Follicular-fluid extracellular vesicles support energy metabolism of bovine oocytes, improving blastocyst development and quality†.Biology of reproduction · 2025Article
- Establishment and culture of mouse oviductal organoids and isolation and characterization of their secreted extracellular vesicles.PloS one · 2025Article
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 6 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Elevated summer temperature is reported to be the leading cause of stress in dairy and beef cows, which negatively affects various reproductive functions. Follicular cells respond to heat stress (HS) by activating the expression of heat shock family proteins (HSPs) and other antioxidants. HS is reported to negatively affect the bi-directional communication between the follicular cells and the oocyte, which is partly mediated by follicular fluid extracellular vesicles (EVs) released from surrounding cells. As carriers of bioactive molecules (DNA, RNA, protein, and lipids), the involvement of EVs in mediating the stress response in follicular cells is not fully understood. Here we used an in vitro model to decipher the cellular and EV-coupled miRNAs of bovine granulosa cells in response to HS. Moreover, the protective role of stress-related EVs against subsequent HS was assessed. For this, bovine granulosa cells from smaller follicles were cultured in vitro and after sub-confluency, cells were either kept at 37 °C or subjected to HS (42 °C). Results showed that granulosa cells exposed to HS increased the accumulation of ROS, total oxidized protein, apoptosis, and the expression of HSPs and antioxidants, while the viability of cells was reduced. Moreover, 14 and 6 miRNAs were differentially expressed in heat-stressed granulosa cells and the corresponding EVs, respectively. Supplementation of stress-related EVs in cultured granulosa cells has induced adaptive response to subsequent HS. However, this potential was not pronounced when the cells were kept under 37 °C. Taking together, EVs generated from granulosa cells exposed to HS has the potential to shuttle bioactive molecules to recipient cells and make them robust to subsequent HS.
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