ArticleNucleic acids research2020
Periphilin self-association underpins epigenetic silencing by the HUSH complex.
Article in Nucleic acids research, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed.
- Article
- LINE-1 Epigenetic Repression and Regulation of Immunity.Viruses · 2026Review
- The HIV-1 restriction factor RPRD2 does not inhibit transcription of HIV-1 or endogenous retroelements.bioRxiv : the preprint server for biology · 2026Article
- Review
- Type I interferons increase expression of endogenous retrovirus K102 and envelope protein in myeloid cells from patients with autoimmune disease.Mobile DNA · 2025Article
- Interferons and Cytokines Induce Transcriptional Activation of the Long-Interspersed Element-1 in Myeloid Cells from Autoimmune Patients.European journal of immunology · 2025Article
- Structure and Methyl-lysine Binding Selectivity of the HUSH Complex Subunit MPP8.Journal of molecular biology · 2025Article
- RNA binding by Periphilin plays an essential role in initiating silencing by the HUSH complex.Nucleic acids research · 2025Article
- Interplay between Two Paralogous Human Silencing Hub (HuSH) Complexes in Regulating LINE-1 Element Silencing.Nature communications · 2024Article
- DNA methylation governs the sensitivity of repeats to restriction by the HUSH-MORC2 corepressor.Nature communications · 2024Article
- Keep quiet: the HUSH complex in transcriptional silencing and disease.Nature structural & molecular biology · 2024Review
- Review
- Article
- The DUF3715 domain has a conserved role in RNA-directed transposon silencing.RNA (New York, N.Y.) · 2023Article
- Co-transcriptional genome surveillance by HUSH is coupled to termination machinery.Molecular cell · 2023Article
- RNA-mediated heterochromatin formation at repetitive elements in mammals.The EMBO journal · 2023Review
- Characterization of Hepatoma-Derived Growth Factor-Related Protein 2 Interactions with Heterochromatin.Cells · 2023Article
- Chromatin modifier HUSH co-operates with RNA decay factor NEXT to restrict transposable element expression.Molecular cell · 2022Article
- A Peptidomimetic Ligand Targeting the Chromodomain of MPP8 Reveals HRP2's Association with the HUSH Complex.ACS chemical biology · 2021Article
- SETDB1-Mediated Silencing of Retroelements.Viruses · 2020Review
Corrections and comments
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Authors and funding
12 authors.
Funding
Abstract
Transcription of integrated DNA from viruses or transposable elements is tightly regulated to prevent pathogenesis. The Human Silencing Hub (HUSH), composed of Periphilin, TASOR and MPP8, silences transcriptionally active viral and endogenous transgenes. HUSH recruits effectors that alter the epigenetic landscape and chromatin structure, but how HUSH recognizes target loci and represses their expression remains unclear. We identify the physicochemical properties of Periphilin necessary for HUSH assembly and silencing. A disordered N-terminal domain (NTD) and structured C-terminal domain are essential for silencing. A crystal structure of the Periphilin-TASOR minimal core complex shows Periphilin forms an α-helical homodimer, bound by a single TASOR molecule. The NTD forms insoluble aggregates through an arginine/tyrosine-rich sequence reminiscent of low-complexity regions from self-associating RNA-binding proteins. Residues required for TASOR binding and aggregation were required for HUSH-dependent silencing and genome-wide deposition of repressive mark H3K9me3. The NTD was functionally complemented by low-complexity regions from certain RNA-binding proteins and proteins that form condensates or fibrils. Our work suggests the associative properties of Periphilin promote HUSH aggregation at target loci.
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Registered trials
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