Evidence map›Paper›PMID 32974600›Full record

ArticleAccess microbiology2020

Whatman FTA cards versus plasma specimens for the quantitation of HIV-1 RNA using two real-time PCR assays.

Abdourahamane Yacouba, Malika Congo, Gérard Komonsira Dioma, Hermann Somlare, David Coulidiaty, Kalifa Ouattara, Lassana Sangare

Open access · goldAbstract read
In one paragraph

Article in Access microbiology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.7field-weighted citation impact, top 33% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 8 citations in OpenAlex.

  1. Article
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  6. Optimizing Release of Nucleic Acids ofInternational journal of molecular sciences · 2021
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Abdourahamane YacoubaLaboratoire National de Référence du VIH/Sida et des Infections Sexuellement Transmissibles, Centre Hospitalier Universitaire Yalgado Ouédraogo, 03 B.P. 7022 Ouaga 03, Ouagadougou, Burkina Faso.ORCID https://orcid.org/0000-0002-0950-2205
Malika CongoLaboratoire National de Référence du VIH/Sida et des Infections Sexuellement Transmissibles, Centre Hospitalier Universitaire Yalgado Ouédraogo, 03 B.P. 7022 Ouaga 03, Ouagadougou, Burkina Faso.
Gérard Komonsira DiomaLaboratoire National de Référence du VIH/Sida et des Infections Sexuellement Transmissibles, Centre Hospitalier Universitaire Yalgado Ouédraogo, 03 B.P. 7022 Ouaga 03, Ouagadougou, Burkina Faso.
Hermann SomlareLaboratoire National de Référence du VIH/Sida et des Infections Sexuellement Transmissibles, Centre Hospitalier Universitaire Yalgado Ouédraogo, 03 B.P. 7022 Ouaga 03, Ouagadougou, Burkina Faso.
David CoulidiatyLaboratoire National de Référence du VIH/Sida et des Infections Sexuellement Transmissibles, Centre Hospitalier Universitaire Yalgado Ouédraogo, 03 B.P. 7022 Ouaga 03, Ouagadougou, Burkina Faso.
Kalifa OuattaraLaboratoire National de Référence du VIH/Sida et des Infections Sexuellement Transmissibles, Centre Hospitalier Universitaire Yalgado Ouédraogo, 03 B.P. 7022 Ouaga 03, Ouagadougou, Burkina Faso.
Lassana SangareLaboratoire National de Référence du VIH/Sida et des Infections Sexuellement Transmissibles, Centre Hospitalier Universitaire Yalgado Ouédraogo, 03 B.P. 7022 Ouaga 03, Ouagadougou, Burkina Faso.
Centre Hospitalier Universitaire Yalgado Ouédraogo · BFUniversité Joseph Ki-Zerbo · BF

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundSeveral studies have compared the use of dried blot spot (DBS) as an alternative to plasma specimens, mainly using Whatman 903 cards as filter paper. The aim of this study was to evaluate the use of Whatman FTA card (FTA card) specimens for HIV-1 viral load testing compared to plasma specimens using two real-time PCR assays manufactured by Roche and Abbott. METHODOLOGY: A cross-sectional study was conducted between April 2017 and September 2017 on HIV-1 patients admitted to Yalgado Ouédraogo Teaching Hospital. Paired FTA cards and plasma specimens were collected and analysed using the Abbott Real-Time HIV-1 assay (Abbott) and COBAS AmpliPrep/COBAS TaqMan v2.0 (Roche).

resultsIn total, 107 patients were included. No statistical differences (

conclusionOur study demonstrates the feasibility of using FTA card filter paper for HIV-1 viral load testing. However, further studies will be required for the validation of the use of FTA card filter paper in HIV-1 treatment monitoring.

Indexed as

Burkina Fasodried blood spotsHIV-1real-time PCRviral loadWhatman FTA cards

Identifiers

PMID32974600
PMCPMC7497827
OpenAlexW3036980925

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.