Evidence map›Paper›PMID 32974570›Full record

ArticleAccess microbiology2020

Laboratory methods supporting measles surveillance in Queensland, Australia, 2010-2017.

Jamie L McMahon, Judith A Northill, Mitchell Finger, Michael Lyon, Stephen B Lambert, Ian M Mackay

Abstract read
In one paragraph

Article in Access microbiology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Jamie L McMahonChild Health Research Centre, The University of Queensland, Brisbane, Queensland, Australia.ORCID https://orcid.org/0000-0002-9304-1500
Judith A NorthillPublic Health Virology Laboratory, Forensic and Scientific Services, Queensland Health, Coopers Plains, Coopers Plains, Queensland, Australia.ORCID https://orcid.org/0000-0002-7549-3343
Mitchell FingerPublic Health Virology Laboratory, Forensic and Scientific Services, Queensland Health, Coopers Plains, Coopers Plains, Queensland, Australia.ORCID https://orcid.org/0000-0002-3861-1443
Michael LyonPublic Health Virology Laboratory, Forensic and Scientific Services, Queensland Health, Coopers Plains, Coopers Plains, Queensland, Australia.
Stephen B LambertChild Health Research Centre, The University of Queensland, Brisbane, Queensland, Australia.ORCID https://orcid.org/0000-0002-2709-193X
Ian M MackayChild Health Research Centre, The University of Queensland, Brisbane, Queensland, Australia.ORCID https://orcid.org/0000-0003-3598-2350

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

purposeAustralia was officially recognised as having eliminated endemic measles transmission in 2014. Maintaining laboratory support for surveillance of vaccine-preventable diseases, such as measles, is an essential component of reaching and maintaining transmission-free status. METHODOLOGY: Real-time and conventional PCR-based tools were used to detect, differentiate from measles vaccine virus (MeVV), and sequence fragments of measles viruses (MeV) identified from specimens collected in Queensland. Specimens were mostly from travellers who had visited or returned to Queensland from international or interstate sites or been in contact with a case from either group.

resultsBetween 2010 and 2017, 13 678 specimens were tested in our laboratory using real-time RT-PCR (RT-rPCR), identifying 533 positives. Most specimens were swabs (70.98 %) and urines (25.56 %). A MeVV RT-rPCR was used on request and identified 154 instances of MeVV. MeV-positive extracts were genotyped as required. Genotypes identified among sequenced specimens included B3, D4, D8, D9, G3, and H1 as well as members of clade A as expected from the detection of MeV among virus introductions due to global travel and vaccination.

conclusionWe describe the workflow employed and results from our laboratory between 2010 and 2017 for the sensitive detection of MeV infection, supporting high-quality surveillance to ensure the maintenance of Australia's measles-free status.

Indexed as

diagnosticsepidemiologymeaslesMeasles virus

Identifiers

PMID32974570
PMCPMC7470308

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.