ArticleFrontiers in bioengineering and biotechnology2020
A Straightforward Assay for Screening and Quantification of Biosurfactants in Microbial Culture Supernatants.
Article in Frontiers in bioengineering and biotechnology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- RhlR quorum-sensing receptor ligand sensitivity regulates the differential expression of phenazine genes inJournal of bacteriology · 2026Article
- Multidomain DNA-Protein Mining Reveals Polymorphic Variations in RhlB Enhancing Monorhamnolipid Biosynthesis.ACS synthetic biology · 2026Article
- Upcycling of polyamides through chemical hydrolysis and engineered Pseudomonas putida.Nature microbiology · 2025Article
- Screening strategies and production of biosurfactants (BSs)/bioemulsifiers (BEs) from marine yeasts and fungi.AIMS microbiology · 2025Review
- Optimisation of surfactin yield inComputational and structural biotechnology journal · 2024Article
- High-throughput evaluation of hemolytic activity through precise measurement of colony and hemolytic zone sizes of engineeredBiology methods & protocols · 2024Article
- Microbial biosurfactants: Green alternatives and sustainable solution for augmenting pesticide remediation and management of organic waste.Current research in microbial sciences · 2024Review
- Simple method for quantification of anionic biosurfactants in aqueous solutions.Frontiers in bioengineering and biotechnology · 2023Article
- Biotechnological potential of microbial bio-surfactants, their significance, and diverse applications.FEMS microbes · 2023Review
- The Glycine-Glucolipid of Alcanivorax borkumensis Is Resident to the Bacterial Cell Wall.Applied and environmental microbiology · 2022Article
- Screening Strategies for Biosurfactant Discovery.Advances in biochemical engineering/biotechnology · 2022Article
- Microbial biosurfactant research: time to improve the rigour in the reporting of synthesis, functional characterization and process development.Microbial biotechnology · 2021Review
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A large variety of microorganisms produces biosurfactants with the potential for a number of diverse industrial applications. To identify suitable wild-type or engineered production strains, efficient screening methods are needed, allowing for rapid and reliable quantification of biosurfactants in multiple cultures, preferably at high throughput. To this end, we have established a novel and sensitive assay for the quantification of biosurfactants based on the dye Victoria Pure Blue BO (VPBO). The assay allows the colorimetric assessment of biosurfactants directly in culture supernatants and does not require extraction or concentration procedures. Working ranges were determined for precise quantification of different rhamnolipid biosurfactants; titers in culture supernatants of recombinant
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