ArticlePlant methods2020
A modified method for enzymatic isolation of and subsequent wax extraction from
Article in Plant methods, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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Who cites it
3 citing papers in PubMed, 19 citations in OpenAlex.
- Hygrometrically controlled programmed cell death drives anther opening and pollen release.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Structure, Assembly and Function of Cuticle from Mechanical Perspective with Special Focus on Perianth.International journal of molecular sciences · 2021Review
- A Guide to Elucidate the Hidden Multicomponent Layered Structure of Plant Cuticles by Raman Imaging.Frontiers in plant science · 2021Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe plant cuticle represents one of the major adaptations of vascular plants to terrestrial life. Cuticular permeability and chemical composition differ among species.
resultsWe developed a method for isolation of thin cuticles that allows reducing the isolation time, the separation of abaxial and adaxial cuticles, and avoids formation of wrinkles. Optical microscopy was used for studying cuticle intactness and scanning electron microscopy for visualisation of external and internal cuticle structures after isolation. Wax extracts were analysed by GC-MS. Isolation of intact cuticle was successful for all tested plants. The wax compositions (very-long-chained fatty acids, alcohols and alkanes) of intact leaves and isolated cuticles of wild type Col-0 were compared.
conclusionsWe conclude that the optimised enzymatic method is suitable for the isolation of
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Registered trials
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