ArticleFrontiers in plant science2020
Application of Aptamers Improves CRISPR-Based Live Imaging of Plant Telomeres.
Article in Frontiers in plant science, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 28 citations in OpenAlex.
- dSaCas9 enables enhanced transcriptional activation in Nicotiana benthamiana compared to its dSpCas9 ortholog.BMC plant biology · 2026Article
- TRB proteins in moss reveal their evolutionarily conserved roles in plant development and telomere maintenance.The Plant journal : for cell and molecular biology · 2025Article
- Real-Time Imaging of Specific Genomic Loci With CRISPR/dCas9 in Human Cells Using CRISPRainbow.Bio-protocol · 2025Article
- CRISPR-CISH: an in situ chromogenic DNA repeat detection system for research and life science education.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2025Article
- Review
- The potential of ALFA-tag and tyramide-based fluorescence signal amplification to expand the CRISPR-based DNA imaging toolkit.Journal of experimental botany · 2024Article
- An optimised CRISPR Cas9 and Cas12a mutagenesis toolkit for Barley and Wheat.Plant methods · 2024Article
- Not Only Editing: A Cas-Cade of CRISPR/Cas-Based Tools for Functional Genomics in Plants and Animals.International journal of molecular sciences · 2024Review
- Engineered aptamers for molecular imaging.Chemical science · 2023Review
- CRISPRi-mediated tunable control of gene expression level with engineered single-guide RNA in Escherichia coli.Nucleic acids research · 2023Article
- CRISPR-Based Genome Editing Tools: An Accelerator in Crop Breeding for a Changing Future.International journal of molecular sciences · 2023Review
- CRISPR-FISH: A CRISPR/Cas9-Based In Situ Labeling Method.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Aptamer-based CRISPR-Cas powered diagnostics of diverse biomarkers and small molecule targets.Applied biological chemistry · 2023Review
- Review
- Hairy CRISPR: Genome Editing in Plants Using Hairy Root Transformation.Plants (Basel, Switzerland) · 2021Review
- CRISPR/dCas9-Based Systems: Mechanisms and Applications in Plant Sciences.Plants (Basel, Switzerland) · 2021Review
- Novel CRISPR/Cas applications in plants: from prime editing to chromosome engineering.Transgenic research · 2021Review
- Caught in the Act: Live-Cell Imaging of Plant Meiosis.Frontiers in plant science · 2021Review
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Development of live imaging techniques for providing information how chromatin is organized in living cells is pivotal to decipher the regulation of biological processes. Here, we demonstrate the improvement of a live imaging technique based on CRISPR/Cas9. In this approach, the sgRNA scaffold is fused to RNA aptamers including MS2 and PP7. When the dead Cas9 (dCas9) is co-expressed with chimeric sgRNA, the fluorescent coat protein-tagged for MS2 and PP7 aptamers (tdMCP-FP and tdPCP-FP) are recruited to the targeted sequence. Compared to previous work with dCas9:GFP, we show that the quality of telomere labeling was improved in transiently transformed
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.