ArticleFrontiers in microbiology2020
The Strengths of Scanning Electron Microscopy in Deciphering SARS-CoV-2 Infectious Cycle.
Article in Frontiers in microbiology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
34 citing papers in PubMed, 70 citations in OpenAlex.
- Fatty acids from Hermetia illucens larvae disrupt membrane integrity in colistin-resistant Acinetobacter baumannii.BMC microbiology · 2026Article
- Article
- Morphological analysis for two types of viral particles in vacuoles of SARS-CoV-2-infected cells.Medical molecular morphology · 2024Article
- Reconstruction of the real 3D shape of the SARS-CoV-2 virus.Biophysical journal · 2024Article
- The unfolded protein response components IRE1α and XBP1 promote human coronavirus infection.mBio · 2023Article
- Modelling the viral dynamics of the SARS-CoV-2 Delta and Omicron variants in different cell types.Journal of the Royal Society, Interface · 2023Article
- Postmortem lung and heart examination of COVID-19 patients in a case series from Jordan.Journal of pathology and translational medicine · 2023Article
- In vitro screening of anti-viral and virucidal effects against SARS-CoV-2 by Hypericum perforatum and Echinacea.Scientific reports · 2022Article
- Near-Native Visualization of SARS-CoV-2 Induced Membrane Remodeling and Virion Morphogenesis.Viruses · 2022Article
- Microscopic observations of SARS-CoV-2 like particles in different oral samples.European journal of oral sciences · 2022Article
- Article
- Article
- Ultrastructural Characterization of Human Bronchial Epithelial Cells during SARS-CoV-2 Infection: Morphological Comparison of Wild-Type and CFTR-Modified Cells.International journal of molecular sciences · 2022Article
- Cell tropism and viral clearance during SARS-CoV-2 lung infection.Pathology, research and practice · 2022Article
- Three-dimensional reconstruction by electron tomography for the application to ultrastructural analysis of SARS-CoV-2 particles.Medical molecular morphology · 2022Article
- Integrative multiomics and in silico analysis revealed the role of ARHGEF1 and its screened antagonist in mild and severe COVID-19 patients.Journal of cellular biochemistry · 2022Article
- Effect of not vortexing nasopharyngeal and throat swabs on SARS-CoV-2 nucleic acid detection: A pilot study.Journal of virological methods · 2022Article
- Limited permissibility of ENL-R and Mv-1-Lu mink cell lines to SARS-CoV-2.Frontiers in microbiology · 2022Article
- Imaging Techniques: Essential Tools for the Study of SARS-CoV-2 Infection.Frontiers in cellular and infection microbiology · 2022Review
- COVID-19: A review of newly formed viral clades, pathophysiology, therapeutic strategies and current vaccination tasks.International journal of biological macromolecules · 2021Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Electron microscopy is a powerful tool in the field of microbiology. It has played a key role in the rapid diagnosis of viruses in patient samples and has contributed significantly to the clarification of virus structure and function, helping to guide the public health response to emerging viral infections. In the present study, we used scanning electron microscopy (SEM) to study the infectious cycle of SARS-CoV-2 in Vero E6 cells and we controlled some key findings by classical transmission electronic microscopy (TEM). The replication cycle of the virus was followed from 1 to 36 h post-infection. Our results revealed that SARS-CoV-2 infected the cells through membrane fusion. Particles are formed in the peri-nuclear region from a budding of the endoplasmic reticulum-Golgi apparatus complex into morphogenesis matrix vesicae. New SARS-CoV-2 particles were expelled from the cells, through cell lysis or by fusion of virus containing vacuoles with the cell plasma membrane. Overall, this cycle is highly comparable to that of SARS-CoV. By providing a detailed and complete SARS-CoV-2 infectious cycle, SEM proves to be a very rapid and efficient tool compared to classical TEM.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.