ArticleScientific reports2020
Inspired by the human placenta: a novel 3D bioprinted membrane system to create barrier models.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 49 citations in OpenAlex.
- A bioengineered model of human placental exposure to environmental metals during pregnancy.Nature biomedical engineering · 2026Article
- Recapitulating gestational progression: a high-throughput 3D bioprinted vascularized human placenta demonstrating stage-specific molecular and functional signatures of human pregnancy.Scientific reports · 2026Article
- Article
- Beyond traditional models: microfluidic technologies for engineering the human placentaFrontiers in bioengineering and biotechnology · 2026Review
- Modeling human placental biology: a review of organoid technologies.Frontiers in cell and developmental biology · 2025Review
- Modeling reproductive and pregnancy-associated tissues using organ-on-chip platforms: challenges, limitations, and the high throughput data frontier.Frontiers in bioengineering and biotechnology · 2025Review
- Digital twin-enhanced three-organ microphysiological system for studying drug pharmacokinetics in pregnant women.Frontiers in pharmacology · 2025Article
- Cost-Effective Bioimpedance Spectroscopy System for Monitoring Syncytialization In Vitro: Experimental and Numerical Validation of BeWo Cell Fusion.Micromachines · 2024Article
- Review
- Article
- Generation of a pancreas derived hydrogel for the culture of hiPSC derived pancreatic endocrine cells.Scientific reports · 2024Article
- Article
- RISING STARS: Approaches to modeling placental function in preeclampsia in vitro and in vivo.The Journal of endocrinology · 2023Article
- Modeling the human placental barrier to understandFrontiers in cellular and infection microbiology · 2023Review
- Principles for optimization and validation of mRNA lipid nanoparticle vaccines against COVID-19 using 3D bioprinting.Nano today · 2022Article
- Registered Clinical Trials Comprising Pregnant Women in China: A Cross-Sectional Study.Frontiers in pharmacology · 2022Article
- Overview of Drug Transporters in Human Placenta.International journal of molecular sciences · 2021Review
- On Placental Toxicology Studies and Cerium Dioxide Nanoparticles.International journal of molecular sciences · 2021Review
- Are the Organoid Models an Invaluable Contribution to ZIKA Virus Research?Pathogens (Basel, Switzerland) · 2021Review
- The Role of the 3Rs for Understanding and Modeling the Human Placenta.Journal of clinical medicine · 2021Review
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Barrier organ models need a scaffold structure to create a two compartment culture. Technical filter membranes used most often as scaffolds may impact cell behaviour and present a barrier themselves, ultimately limiting transferability of test results. In this work we present an alternative for technical filter membrane systems: a 3D bioprinted biological membrane in 24 well format. The biological membrane, based on extracellular matrix (ECM), is highly permeable and presents a natural 3D environment for cell culture. Inspired by the human placenta we established a coculture of a trophoblast-derived cell line (BeWo b30), together with primary placental fibroblasts within the biological membrane (simulating villous stroma) and primary human placental endothelial cells-representing three cellular components of the human placental villus. All cell types maintained their cell type specific marker expression after two weeks of coculture on the biological membrane. In permeability assays the trophoblast layer developed a barrier on the biological membrane, which was even more pronounced when cocultured with fibroblasts. In this work we present a filter membrane free scaffold, we characterize its properties and assess its suitability for cell culture and barrier models. Further we show a novel placenta inspired model in a complex bioprinted coculture. In the absence of an artificial filter membrane, we demonstrate barrier architecture and functionality.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.