ArticleScientific reports2020
Generation of myostatin edited horse embryos using CRISPR/Cas9 technology and somatic cell nuclear transfer.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 44 citations in OpenAlex.
- Advances in Equine Genomics: Decoding the Genetic Architecture of Morphology, Performance, Behavior, and Adaptation.Molecular biotechnology · 2026Review
- Equine models in translational medicine: A comparative approach to human health.Animal models and experimental medicine · 2026Review
- Cell and Gene Therapy in Equine Ocular Disease.Veterinary ophthalmology · 2026Review
- Production of gene-edited cloned cattle embryos using the CRISPR/EOCas12i system.Frontiers in genome editing · 2026Article
- Genome editing between wonder and rejection.Frontiers in bioengineering and biotechnology · 2026Article
- Osmotically Tunable Microdroplets Enable Amplification-Free CRISPR Detection of Gene Doping.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Cytosine base editor-mediated high-efficiency myostatin editing in Hu sheep.Functional & integrative genomics · 2025Article
- Gene doping in horse racing and equine sports: Current landscape and future perspectives.Equine veterinary journal · 2025Review
- Opportunities for CRISPR-Cas9 application in farm animal genetic improvement.Molecular biology reports · 2024Review
- CRISPR-Cas guide RNA indel analysis using CRISPResso2 with Nanopore sequencing data.BMC research notes · 2024Article
- CRISPR-mediated editing of β-lactoglobulin (BLG) gene in buffalo.Scientific reports · 2024Article
- Efficient and Specific Generation ofGenes · 2023Article
- Short Insertion and Deletion Discoveries via Whole-Genome Sequencing of 101 Thoroughbred Racehorses.Genes · 2023Article
- Conditionally immortalised equine skeletal muscle cell lines forBiochemistry and biophysics reports · 2023Article
- First sex modification case in equine cloning.PloS one · 2023Article
- Article
- Article
- Control of gene doping in human and horse sports.Gene therapy · 2022Article
- Oocyte Penetration Speed Optimization Based on Intracellular Strain.Micromachines · 2022Article
- HLA-G gene editing in tumor cell lines as a novel alternative in cancer immunotherapy.Scientific reports · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The application of new technologies for gene editing in horses may allow the generation of improved sportive individuals. Here, we aimed to knock out the myostatin gene (MSTN), a negative regulator of muscle mass development, using CRISPR/Cas9 and to generate edited embryos for the first time in horses. We nucleofected horse fetal fibroblasts with 1, 2 or 5 µg of 2 different gRNA/Cas9 plasmids targeting the first exon of MSTN. We observed that increasing plasmid concentrations improved mutation efficiency. The average efficiency was 63.6% for gRNA1 (14/22 edited clonal cell lines) and 96.2% for gRNA2 (25/26 edited clonal cell lines). Three clonal cell lines were chosen for embryo generation by somatic cell nuclear transfer: one with a monoallelic edition, one with biallelic heterozygous editions and one with a biallelic homozygous edition, which rendered edited blastocysts in each case. Both MSTN editions and off-targets were analyzed in the embryos. In conclusion, CRISPR/Cas9 proved an efficient method to edit the horse genome in a dose dependent manner with high specificity. Adapting this technology sport advantageous alleles could be generated, and a precision breeding program could be developed.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.