Evidence map›Paper›PMID 32973160›Full record

ArticleNature communications2020

Functional genetic encoding of sulfotyrosine in mammalian cells.

Xinyuan He, Yan Chen, Daisy Guiza Beltran, Maia Kelly, Bin Ma, Justin Lawrie, Feng Wang, Eric Dodds, Limei Zhang, Jiantao Guo and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
26citing papers in PubMed, 1 pooled it
7.1field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

26 citing papers in PubMed, 1 synthesis or guideline pooled it, 64 citations in OpenAlex.

  1. Pooled it
  2. Review
  3. In Vivo Biosynthesis and Direct Incorporation of Noncanonical Amino Acids into Proteins.Chembiochem : a European journal of chemical biology · 2025
    Review
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  6. Review
  7. Article
  8. Review
  9. Review
  10. Article
  11. Review
  12. Review
  13. Review
  14. Fluorosulfate as a Latent Sulfate in Peptides and Proteins.Journal of the American Chemical Society · 2023
    Article
  15. Article
  16. Article
  17. Article
  18. Review
  19. Site-Specific Incorporation of Sulfotyrosine into Proteins in Mammalian Cells.Methods in molecular biology (Clifton, N.J.) · 2023
    Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 2 institutions in 2 countries.

Xinyuan HeDepartment of Chemical & Biomolecular Engineering, University of Nebraska-Lincoln, Lincoln, Nebraska, 68588, USA.
Yan Chen *Department of Chemistry, University of Nebraska-Lincoln, Lincoln, Nebraska, 68588, USA.
Daisy Guiza Beltran *Department of Biochemistry, University of Nebraska-Lincoln, Lincoln, Nebraska, 68588, USA.
Maia Kelly *Department of Chemistry, University of Nebraska-Lincoln, Lincoln, Nebraska, 68588, USA.ORCID http://orcid.org/0000-0002-9383-1107
Bin MaDepartment of Chemistry, University of Nebraska-Lincoln, Lincoln, Nebraska, 68588, USA.
Justin LawrieDepartment of Chemistry, University of Nebraska-Lincoln, Lincoln, Nebraska, 68588, USA.
Feng WangInstitute of Biophysics, Chinese Academy of Sciences, Beijing, China.ORCID http://orcid.org/0000-0003-2980-4646
Eric DoddsDepartment of Chemistry, University of Nebraska-Lincoln, Lincoln, Nebraska, 68588, USA.
Limei ZhangDepartment of Biochemistry, University of Nebraska-Lincoln, Lincoln, Nebraska, 68588, USA.ORCID http://orcid.org/0000-0001-6807-5103
Jiantao GuoDepartment of Chemistry, University of Nebraska-Lincoln, Lincoln, Nebraska, 68588, USA. jguo4@unl.edu.ORCID http://orcid.org/0000-0001-6983-9953
Wei NiuDepartment of Chemical & Biomolecular Engineering, University of Nebraska-Lincoln, Lincoln, Nebraska, 68588, USA. wniu2@unl.edu.ORCID http://orcid.org/0000-0003-3826-1276
University of Nebraska–Lincoln · USChinese Academy of Sciences · CN

Funding

Redox Biology CenterP30GM103335 · NIGMS · UNIVERSITY OF NEBRASKA LINCOLN · PI BECKER, DONALD F · 2012 to 2016
$4.3M
Improve the safety of an efficacious live-attenuated HIV-1 vaccine through unnatuR01AI111862 · NIAID · UNIVERSITY OF NEBRASKA LINCOLN · PI GUO, JIANTAO, LI, QINGSHENG · 2014 to 2017
$1.9M
NIAID NIH HHS R01 AI111862NIGMS NIH HHS P30 GM103335
6 · The paper itself

Abstract

Protein tyrosine O-sulfation (PTS) plays a crucial role in extracellular biomolecular interactions that dictate various cellular processes. It also involves in the development of many human diseases. Regardless of recent progress, our current understanding of PTS is still in its infancy. To promote and facilitate relevant studies, a generally applicable method is needed to enable efficient expression of sulfoproteins with defined sulfation sites in live mammalian cells. Here we report the engineering, in vitro biochemical characterization, structural study, and in vivo functional verification of a tyrosyl-tRNA synthetase mutant for the genetic encoding of sulfotyrosine in mammalian cells. We further apply this chemical biology tool to cell-based studies on the role of a sulfation site in the activation of chemokine receptor CXCR4 by its ligand. Our work will not only facilitate cellular studies of PTS, but also paves the way for economical production of sulfated proteins as therapeutic agents in mammalian systems.

Indexed as

AnimalsChemokinesCRISPR-Cas SystemsCrystallography, X-RayGene Knockout TechniquesHEK293 CellsHumansLigandsModels, MolecularProtein ConformationReceptors, CXCR4TyrosineTyrosine-tRNA LigaseChemokinesCXCR4 protein, humanLigandsReceptors, CXCR4Tyrosinetyrosine O-sulfateTyrosine-tRNA Ligase

Identifiers

PMID32973160
PMCPMC7515910
OpenAlexW3089106521

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.