ArticleInternational journal of molecular sciences2020
Alternating Electric Fields Modify the Function of Human Osteoblasts Growing on and in the Surroundings of Titanium Electrodes.
Article in International journal of molecular sciences, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 31 citations in OpenAlex.
- Advances and Challenges in Constructing Bone Organoids Using Cells Derived from Human Pluripotent Stem Cells: A Review.Stem cell reviews and reports · 2026Review
- Parameter-resolved AC electrical stimulation links electrical system characteristics to early transcriptional responses in human stem cells.Frontiers in bioengineering and biotechnology · 2026Article
- Enhancing Bone Health with Conjugated Linoleic Acid: Mechanisms, Challenges, and Innovative Strategies.Nutrients · 2025Review
- Safety and preliminary efficacy of an electrically stimulated implant for mandibular bone regeneration: a pilot study in a large animal model.Clinical oral investigations · 2025Article
- Article
- Unleashing the Potential of Electroactive Hybrid Biomaterials and Self-Powered Systems for Bone Therapeutics.Nano-micro letters · 2024Review
- Advances in electroactive biomaterials: Through the lens of electrical stimulation promoting bone regeneration strategy.Journal of orthopaedic translation · 2024Review
- The effect of titanium surface treatment by application of constant potential or current on the viability of pre-osteoblast cells: anFrontiers in bioengineering and biotechnology · 2024Article
- Discrimination between the effects of pulsed electrical stimulation and electrochemically conditioned medium on human osteoblasts.Journal of biological engineering · 2023Article
- Article
- Researching progress on bio-reactive electrogenic materials with electrophysiological activity for enhanced bone regeneration.Frontiers in bioengineering and biotechnology · 2022Review
- Long-term stimulation with alternating electric fields modulates the differentiation and mineralization of human pre-osteoblasts.Frontiers in physiology · 2022Article
- Conductive Scaffolds for Bone Tissue Engineering: Current State and Future Outlook.Journal of functional biomaterials · 2021Review
- Anodic TiONanomaterials (Basel, Switzerland) · 2021Review
- Properties improvement of titanium alloys scaffolds in bone tissue engineering: a literature review.Annals of translational medicine · 2021Review
- Article
- Impact of exogenous direct electric current on the expression of mRNA related to OPG, in SaOS-2 cells using quantitative reverse transcription polymerase chain reaction: A qualitative and quantitative analysis.Journal of oral biology and craniofacial researchArticle
- Optimizing Alternating Current Electrical Stimulation Parameters to Enhance Osteoblasts Differentiation.BioFactors (Oxford, England)Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Endogenous electric fields created in bone tissue as a response to mechanical loading are known to influence the activity and differentiation of bone and precursor cells. Thus, electrical stimulation offers an adjunct therapy option for the promotion of bone regeneration. Understanding the influence of electric fields on bone cell function and the identification of suitable electrical stimulation parameters are crucial for the clinical success of stimulation therapy. Therefore, we investigated the impact of alternating electric fields on human osteoblasts that were seeded on titanium electrodes, which delivered the electrical stimulation. Moreover, osteoblasts were seeded on collagen-coated coverslips near the electrodes, representing the bone stock surrounding the implant. Next, 0.2 V, 1.4 V, or 2.8 V were applied to the in vitro system with 20 Hz frequency. After one, three, and seven days, the osteoblast morphology and expression of osteogenic genes were analysed. The actin organisation, as well as the proliferation, were not affected by the electrical stimulation. Changes in the gene expression and protein accumulation after electrical stimulation were voltage-dependent. After three days, the osteogenic gene expression and alkaline phosphatase activity were up to 2.35-fold higher following the electrical stimulation with 0.2 V and 1.4 V on electrodes and coverslips compared to controls. Furthermore, collagen type I mRNA, as well as the amount of the C-terminal propeptide of collagen type I were increased after the stimulation with 0.2 V and 1.4 V, while the higher electrical stimulation with 2.8 V led to decreased levels, especially on the electrodes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.