ArticleScientific reports2020
Injectable pre-cultured tissue modules catalyze the formation of extensive functional microvasculature in vivo.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 21 citations in OpenAlex.
- Acoustic cell patterning reveals geometry- and substrate-dependent vasculogenesis and human embryo model development.Nature communications · 2026Article
- Clickable PEG-norbornene microgels support suspension bioprinting and microvascular assembly.Acta biomaterialia · 2025Article
- Biofabrication and Characterization of Vascularizing PEG-Norbornene Microgels.Journal of biomedical materials research. Part A · 2025Article
- Developing Porous Fibrin Scaffolds with Tunable Anisotropic Features to Direct Myoblast Orientation.Tissue engineering. Part C, Methods · 2024Article
- Pre-cultured, cell-encapsulating fibrin microbeads for the vascularization of ischemic tissues.Journal of biomedical materials research. Part A · 2024Article
- Long-term day-by-day tracking of microvascular networks sprouting in fibrin gels: From detailed morphological analyses to general growth rules.APL bioengineering · 2024Article
- Longitudinal Monitoring of Angiogenesis in a Murine Window Chamber ModelTissue engineering. Part C, Methods · 2024Article
- Engineering primitive multiscale chimeric vasculature by combining human microvessels with explanted murine vessels.Scientific reports · 2024Article
- Manufacturing the multiscale vascular hierarchy: progress toward solving the grand challenge of tissue engineering.Trends in biotechnology · 2023Review
- A combination of matrix stiffness and degradability dictate microvascular network assembly and remodeling in cell-laden poly(ethylene glycol) hydrogels.Biomaterials · 2023Article
- Multimodular vascularized bone construct comprised of vasculogenic and osteogenic microtissues.Biotechnology and bioengineering · 2022Article
- Coculture of Endothelial and Stromal Cells to Promote Concurrent Osteogenesis and Vasculogenesis.Tissue engineering. Part A · 2021Article
- Prevascularized Micro-/Nano-Sized Spheroid/Bead Aggregates for Vascular Tissue Engineering.Nano-micro letters · 2021Article
- Spatially-directed angiogenesis using ultrasound-controlled release of basic fibroblast growth factor from acoustically-responsive scaffolds.Acta biomaterialia · 2021Article
- Trabecular bone organoids: a micron-scale 'humanised' prototype designed to study the effects of microgravity and degeneration.NPJ microgravity · 2021Article
Corrections and comments
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
Abstract
Revascularization of ischemic tissues is a major barrier to restoring tissue function in many pathologies. Delivery of pro-angiogenic factors has shown some benefit, but it is difficult to recapitulate the complex set of factors required to form stable vasculature. Cell-based therapies and pre-vascularized tissues have shown promise, but the former require time for vascular assembly in situ while the latter require invasive surgery to implant vascularized scaffolds. Here, we developed cell-laden fibrin microbeads that can be pre-cultured to form primitive vascular networks within the modular structures. These microbeads can be delivered in a minimally invasive manner and form functional microvasculature in vivo. Microbeads containing endothelial cells and stromal fibroblasts were pre-cultured for 3 days in vitro and then injected within a fibrin matrix into subcutaneous pockets on the dorsal flanks of SCID mice. Vessels deployed from these pre-cultured microbeads formed functional connections to host vasculature within 3 days and exhibited extensive, mature vessel coverage after 7 days in vivo. Cellular microbeads showed vascularization potential comparable to bulk cellular hydrogels in this pilot study. Furthermore, our findings highlight some potentially advantageous characteristics of pre-cultured microbeads, such as volume preservation and vascular network distribution, which may be beneficial for treating ischemic diseases.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.