ArticleNature communications2020
A lysozyme with altered substrate specificity facilitates prey cell exit by the periplasmic predator Bdellovibrio bacteriovorus.
Article in Nature communications, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
- Comparison of Predatory Phenotypes and Genotypes Between Bdellovibrio sp. BIS2 and Bacteriovorax sp. HI3 Isolated From the Same Freshwater Environment.Environmental microbiology · 2026Article
- Enrichment isolation and metabolic characteristics ofApplied and environmental microbiology · 2025Article
- The Microbial Community Succession Drives Stage-Specific Carbon Metabolic Shifts DuringMicroorganisms · 2025Article
- Curli-independent defense againstMicrobiology spectrum · 2025Article
- A molecular toolbox to modulate gene expression and protein secretion in the bacterial predator Bdellovibrio bacteriovorus.PLoS genetics · 2025Article
- Quantitative proteome of bacterial periplasmic predation by Bdellovibrio bacteriovorus reveals a prey-lytic protease.Communications biology · 2025Article
- Strategies and mechanisms of contact-dependent predation in bacteria.Current opinion in microbiology · 2025Review
- Article
- Article
- How do Gram-negative bacteria escape predation by Bdellovibrio bacteriovorus?npj antimicrobials and resistance · 2024Review
- Bdellovibrio bacteriovorus uses chimeric fibre proteins to recognize and invade a broad range of bacterial hosts.Nature microbiology · 2024Article
- Histones with an unconventional DNA-binding mode in vitro are major chromatin constituents in the bacterium Bdellovibrio bacteriovorus.Nature microbiology · 2023Article
- A genome catalogue of lake bacterial diversity and its drivers at continental scale.Nature microbiology · 2023Article
- Cell cycle-dependent organization of a bacterial centromere through multi-layered regulation of the ParABS system.PLoS genetics · 2023Article
- Moving toward a better understanding of the model bacterial predatorMicrobiology (Reading, England) · 2023Article
- Bdellovibrio predation cycle characterized at nanometre-scale resolution with cryo-electron tomography.Nature microbiology · 2023Article
- Learning with Bdellovibrio.Nature microbiology · 2023Article
- An MltA-Like Lytic Transglycosylase Secreted by Bdellovibrio bacteriovorus Cleaves the Prey Septum during Predatory Invasion.Journal of bacteriology · 2023Article
- Parallel Evolution in PredatoryApplied and environmental microbiology · 2023Article
- Advances in cellular and molecular predatory biology ofFrontiers in microbiology · 2023Review
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10 authors.
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Abstract
Lysozymes are among the best-characterized enzymes, acting upon the cell wall substrate peptidoglycan. Here, examining the invasive bacterial periplasmic predator Bdellovibrio bacteriovorus, we report a diversified lysozyme, DslA, which acts, unusually, upon (GlcNAc-) deacetylated peptidoglycan. B. bacteriovorus are known to deacetylate the peptidoglycan of the prey bacterium, generating an important chemical difference between prey and self walls and implying usage of a putative deacetyl-specific "exit enzyme". DslA performs this role, and ΔDslA strains exhibit a delay in leaving from prey. The structure of DslA reveals a modified lysozyme superfamily fold, with several adaptations. Biochemical assays confirm DslA specificity for deacetylated cell wall, and usage of two glutamate residues for catalysis. Exogenous DslA, added ex vivo, is able to prematurely liberate B. bacteriovorus from prey, part-way through the predatory lifecycle. We define a mechanism for specificity that invokes steric selection, and use the resultant motif to identify wider DslA homologues.
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