ArticleScience advances2020
Mechanical stimulation of single cells by reversible host-guest interactions in 3D microscaffolds.
Article in Science advances, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed.
- Modeling mechanochemical coupling in optogenetically activated cell layers.Biophysical journal · 2026Article
- Rational Design and Functionalization of Melt Electrowritten 4D Scaffolds for Biomedical Applications.Nano-micro letters · 2026Review
- Exploring 4D Printing Technology for Biomedical Applications.Current pharmaceutical design · 2026Article
- Dynamic Regulation of Granular Hydrogels Through Guest-Host Interactions to Spatiotemporally Guide Cellular Migration.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Autonomous Implants.Advanced materials (Deerfield Beach, Fla.) · 2025Review
- Fabrication of Site-Specific 3D Structures via Macroscopic Supramolecular Assembly for Spatially Controlled Alignment of Multiple Cells.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Architecture mechanics mediated osteogenic progression in bone regeneration of artificial scaffolds.Science advances · 2025Article
- Femtosecond-Laser Preparation of Hydrogel with Micro/Nano-Structures and their Biomedical Applications.Small science · 2025Review
- 4D Printing of Magnetically Responsive Materials and Their Applications.Research (Washington, D.C.) · 2025Review
- Site-Selective Biofunctionalization of 3D Microstructures Via Direct Ink Writing.Small (Weinheim an der Bergstrasse, Germany) · 2024Article
- Reversible Host-Guest Crosslinks in Supramolecular Hydrogels for On-Demand Mechanical Stimulation of Human Mesenchymal Stem Cells.Advanced healthcare materials · 2024Article
- Article
- Electroactive 4D Porous Scaffold Based on Conducting Polymer as a Responsive and DynamicACS applied materials & interfaces · 2024Article
- Force propagation between epithelial cells depends on active coupling and mechano-structural polarization.eLife · 2023Article
- Advanced Mechanical Testing Technologies at the Cellular Level: The Mechanisms and Application in Tissue Engineering.Polymers · 2023Review
- Auxeticity as a Mechanobiological Tool to Create Meta-Biomaterials.ACS applied bio materials · 2023Review
- Cucurbit[8]uril-based water-dispersible assemblies with enhanced optoacoustic performance for multispectral optoacoustic imaging.Nature communications · 2023Article
- 3D Printed Acoustically Programmable Soft Microactuators.Soft robotics · 2023Article
- Cell size and actin architecture determine force generation in optogenetically activated cells.Biophysical journal · 2023Article
- Review on Bioinspired Design of ECM-Mimicking Scaffolds by Computer-Aided Assembly of Cell-Free and Cell Laden Micro-Modules.Journal of functional biomaterials · 2023Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
13 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Many essential cellular processes are regulated by mechanical properties of their microenvironment. Here, we introduce stimuli-responsive composite scaffolds fabricated by three-dimensional (3D) laser lithography to simultaneously stretch large numbers of single cells in tailored 3D microenvironments. The key material is a stimuli-responsive photoresist containing cross-links formed by noncovalent, directional interactions between β-cyclodextrin (host) and adamantane (guest). This allows reversible actuation under physiological conditions by application of soluble competitive guests. Cells adhering in these scaffolds build up initial traction forces of ~80 nN. After application of an equibiaxial stretch of up to 25%, cells remodel their actin cytoskeleton, double their traction forces, and equilibrate at a new dynamic set point within 30 min. When the stretch is released, traction forces gradually decrease until the initial set point is retrieved. Pharmacological inhibition or knockout of nonmuscle myosin 2A prevents these adjustments, suggesting that cellular tensional homeostasis strongly depends on functional myosin motors.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.