Evidence map›Paper›PMID 32967608›Full record

ArticleBMC genomics2020

Metabolic and transcriptome responses of RNAi-mediated AMPKα knockdown in Tribolium castaneum.

Heng Jiang, Nan Zhang, Caihong Ji, Xiangkun Meng, Kun Qian, Yang Zheng, Jianjun Wang

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC genomics, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
0.6field-weighted citation impact, top 34% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 18 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Heng JiangCollege of Horticulture and Plant Protection, Yangzhou University, Yangzhou, 225009, China.
Nan ZhangCollege of Horticulture and Plant Protection, Yangzhou University, Yangzhou, 225009, China.
Caihong JiCollege of Horticulture and Plant Protection, Yangzhou University, Yangzhou, 225009, China.
Xiangkun MengCollege of Horticulture and Plant Protection, Yangzhou University, Yangzhou, 225009, China.
Kun QianCollege of Horticulture and Plant Protection, Yangzhou University, Yangzhou, 225009, China.
Yang ZhengCollege of Horticulture and Plant Protection, Yangzhou University, Yangzhou, 225009, China.
Jianjun WangCollege of Horticulture and Plant Protection, Yangzhou University, Yangzhou, 225009, China. wangjj@yzu.edu.cn.
Yangzhou University · CN

Funding

National Natural Science Foundation of China 31871974 and 31572000
6 · The paper itself

Abstract

backgroundThe AMP-activated protein kinase (AMPK) is an intracellular fuel sensor for lipid and glucose metabolism. In addition to the short-term regulation of metabolic enzymes by phosphorylation, AMPK may also exert long-term effects on the transcription of downstream genes through the regulation of transcription factors and coactivators. In this study, RNA interference (RNAi) was conducted to investigate the effects of knockdown of TcAMPKα on lipid and carbohydrate metabolism in the red flour beetle, Tribolium castaneum, and the transcriptome profiles of dsTcAMPKα-injected and dsEGFP-injected beetles under normal conditions were compared by RNA-sequencing.

resultsRNAi-mediated suppression of TcAMPKα increased whole-body triglyceride (TG) level and the ratio between glucose and trehalose, as was confirmed by in vivo treatment with the AMPK-activating compound, 5-Aminoimidazole-4-carboxamide1-β-D-ribofuranoside (AICAR). A total of 1184 differentially expressed genes (DEGs) were identified between dsTcAMPKα-injected and dsEGFP-injected beetles. These include genes involved in lipid and carbohydrate metabolism as well as insulin/insulin-like growth factor signaling (IIS). Real-time quantitative polymerase chain reaction analysis confirmed the differential expression of selected genes. Interestingly, metabolism-related transcription factors such as sterol regulatory element-binding protein 1 (SREBP1) and carbohydrate response element-binding protein (ChREBP) were also significantly upregulated in dsTcAMPKα-injected beetles.

conclusionsAMPK plays a critical role in the regulation of beetle metabolism. The findings of DEGs involved in lipid and carbohydrate metabolism provide valuable insight into the role of AMPK signaling in the transcriptional regulation of insect metabolism.

Indexed as

MetabolomeTranscriptomeAMP-Activated Protein Kinase KinasesAnimalsGlucoseInsect ProteinsInsulinProtein KinasesSomatomedinsSterol Regulatory Element Binding ProteinsTrehaloseTriboliumTriglyceridesAMP-Activated Protein Kinase KinasesGlucoseInsect ProteinsInsulinProtein KinasesSomatomedinsSterol Regulatory Element Binding ProteinsTrehaloseTriglyceridesAMPKCarbohydrate metabolism IIS pathwayLipid metabolismRNA interferenceTranscriptomeTribolium castaneum

Identifiers

PMID32967608
PMCPMC7510082
OpenAlexW3089276823

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.