ArticlePathobiology : journal of immunopathology, molecular and cellular biology2020
Application of "Tissueoid Cell Culture System" Using a Silicate Fiber Scaffold for Cancer Research.
Article in Pathobiology : journal of immunopathology, molecular and cellular biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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10 citing papers in PubMed, 12 citations in OpenAlex.
- Cancer cell dynamics on silica fibers.Scientific reports · 2025Article
- Metabolic Analysis of Three-Dimensional Cultured Gastrointestinal Cancer Cells Suggests that <sc>l</sc>-Arginine Inhibits Tumor Growth by Affecting the Urea Cycle.Pathobiology : journal of immunopathology, molecular and cellular biology · 2025Article
- Three-Dimensional Culture of Glioblastoma Cells Using a Tissueoid Cell Culture System.Acta histochemica et cytochemica · 2024Article
- Fabrication and Optimization of 3D-Printed Silica Scaffolds for Neural Precursor Cell Cultivation.Journal of functional biomaterials · 2023Article
- Collagen for neural tissue engineering: Materials, strategies, and challenges.Materials today. Bio · 2023Review
- Cocktail Formula and Application Prospects for Oral and Maxillofacial Organoids.Tissue engineering and regenerative medicine · 2022Review
- Desmosomes in squamous cell carcinomas.Upsala journal of medical sciences · 2022Article
- Differences in the Central Energy Metabolism of Cancer Cells between Conventional 2D and Novel 3D Culture Systems.International journal of molecular sciences · 2021Article
- Similarities and differences in metabolites of tongue cancer cells among two- and three-dimensional cultures and xenografts.Cancer science · 2021Article
- Advancement of Scaffold-Based 3D Cellular Models in Cancer Tissue Engineering: An Update.Frontiers in oncology · 2021Review
Corrections and comments
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10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundWe developed a 3-dimensional (3D) culture system using a high-purity silica fiber scaffold of unwoven sheets called CellbedTM.
methodsWe used adherent colon and esophagogastric junction adenocarcinoma cells, tongue squamous cell carcinoma (SqCC) cells, and nonadherent gastric cancer cells. These cells were subjected to staining with various substances and observed by electron microscopy. To evaluate the effects of extracellular matrix in carcinoma tissues, SqCC cells were cultured in Cellbed coated with collagens I, III, and IV.
resultsEspecially well-differentiated carcinoma cells cultured in this 3D system showed their own unique characteristics: luminal formation in adenocarcinoma cells and cell stratification and keratinization in SqCC cells. Scanning electron microscopy revealed the proliferation of cancer cells with cytoplasm entwined in Cellbed. Intercellular desmosomes in squamous epithelia were detected by transmission electron microscopy of vertical cross sections. SqCC cells cultured in Cellbed coated with collagen IV showed enhanced invasive and proliferative abilities.
conclusionBecause the morphology of cancer cells cultured in this 3D culture system is similar to that in living organisms, we called the system a "tissueoid cell culture system." Coating with collagen IV enables the modification of cell-matrix interactions as well as recapitulation of the in vivo microenvironment.
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