ArticleeLife2020
Stoichiometric interactions explain spindle dynamics and scaling across 100 million years of nematode evolution.
Article in eLife, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Power-law scaling of mitotic spindles with genome sizes across eukaryotes is driven by chromosome crowding.Nature cell biology · 2026Article
- Towards a systems-level view of the microtubule cytoskeleton and its functions in physiology and disease.Nature reviews. Molecular cell biology · 2026Review
- Cell size reduction distinctly scales spindle elongation and chromosome segregation in C. elegans.Nature communications · 2026Article
- Microtubule stiffening by the doublecortin-domain protein ZYG-8 contributes to mitotic spindle orientation during zygote division in Caenorhabditis elegans.PLoS genetics · 2026Article
- A fluorescence lifetime separation approach for FLIM live-cell imaging.Journal of microscopy · 2026Article
- Variation in inbreeding depression within and among Caenorhabditis species.G3 (Bethesda, Md.) · 2025Article
- Cell size reduction scales spindle elongation but not chromosome segregation inResearch square · 2025Article
- Cell size reduction scales spindle elongation but not chromosome segregation inbioRxiv : the preprint server for biology · 2025Article
- Microtubule-driven cell shape changes and actomyosin flow synergize to position the centrosome.The Journal of cell biology · 2025Article
- Quantitative perturbation-phenotype maps reveal nonlinear responses underlying robustness of PAR-dependent asymmetric cell division.PLoS biology · 2024Article
- Live-cell imaging under centrifugation characterized the cellular force for nuclear centration in theProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Multivalent coiled-coil interactions enable full-scale centrosome assembly and strength.The Journal of cell biology · 2024Article
- Article
- Live-cell imaging under centrifugation characterized the cellular force for nuclear centration in thebioRxiv : the preprint server for biology · 2024Article
- Enucleation of theLife science alliance · 2024Article
- Interrelated effects of chromosome size, mechanics, number, location-orientation and polar ejection force on the spindle accuracy: a 3D computational study.Molecular biology of the cell · 2023Article
- Regulation of organelle size and organization during development.Seminars in cell & developmental biology · 2023Review
- Article
- CellDynaMo-stochastic reaction-diffusion-dynamics model: Application to search-and-capture process of mitotic spindle assembly.PLoS computational biology · 2022Article
- Evolutionary divergence of anaphase spindle mechanics in nematode embryos constrained by antagonistic pulling and viscous forces.Molecular biology of the cell · 2022Article
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
The spindle shows remarkable diversity, and changes in an integrated fashion, as cells vary over evolution. Here, we provide a mechanistic explanation for variations in the first mitotic spindle in nematodes. We used a combination of quantitative genetics and biophysics to rule out broad classes of models of the regulation of spindle length and dynamics, and to establish the importance of a balance of cortical pulling forces acting in different directions. These experiments led us to construct a model of cortical pulling forces in which the stoichiometric interactions of microtubules and force generators (each force generator can bind only one microtubule), is key to explaining the dynamics of spindle positioning and elongation, and spindle final length and scaling with cell size. This model accounts for variations in all the spindle traits we studied here, both within species and across nematode species spanning over 100 million years of evolution.
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