ArticleJournal of the American Chemical Society2020
TRPswitch-A Step-Function Chemo-optogenetic Ligand for the Vertebrate TRPA1 Channel.
Article in Journal of the American Chemical Society, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed, 45 citations in OpenAlex.
- TRPA1 Channels as Multimodal Environmental Sensors: Structure-Function Insights From Aquatic Organisms.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2026Review
- Orchestrating photoswitch thermal backconversion using hydrogen bonds to reshape energy landscapes.Nature communications · 2026Article
- Calcium Signaling in Macrophages During a Wound Response In Vivo.International journal of molecular sciences · 2026Article
- Ideal efficacy photoswitching for chromocontrol of TRPC4/5 channel functions in live tissues.Nature chemical biology · 2026Article
- Physiological functions and pharmacological targeting of transient receptor potential channels.Pharmacological reviews · 2025Review
- ON-OFF nanopores for optical control of transmembrane ionic communication.Nature nanotechnology · 2025Article
- A Multimodal, In Vivo Approach for Assessing Structurally and Phenotypically Related Neuroactive Molecules.ACS chemical neuroscience · 2024Article
- Photoswitchable TRPC6 channel activators evoke distinct channel kinetics reflecting different gating behaviors.iScience · 2024Article
- Remote neurostimulation through an endogenous ion channel using a near-infrared light-activatable nanoagonist.Science advances · 2024Article
- Article
- Photoactivatable Nanobody Conjugate Dimerizer Temporally Resolves Tiam1-Rac1 Signaling Axis.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Optogenetics Neuromodulation of the Nose.Behavioural neurology · 2024Review
- BTDAzo: A Photoswitchable TRPC5 Channel Activator.Angewandte Chemie (International ed. in English) · 2022Article
- Atomistic mechanisms of human TRPA1 activation by electrophile irritants through molecular dynamics simulation and mutual information analysis.Scientific reports · 2022Article
- Bistable Photoswitch Allows in Vivo Control of Hematopoiesis.ACS central science · 2022Article
- Optical Assessment of Nociceptive TRP Channel Function at the Peripheral Nerve Terminal.International journal of molecular sciences · 2021Review
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
Chemo-optogenetics has produced powerful tools for optical control of cell activity, but current tools suffer from a variety of limitations including low unitary conductance, the need to modify the target channel, or the inability to control both on and off switching. Using a zebrafish behavior-based screening strategy, we discovered "TRPswitch", a photoswitchable nonelectrophilic ligand scaffold for the transient receptor potential ankyrin 1 (TRPA1) channel. TRPA1 exhibits high unitary channel conductance, making it an ideal target for chemo-optogenetic tool development. Key molecular determinants for the activity of TRPswitch were elucidated and allowed for replacement of the TRPswitch azobenzene with a next-generation azoheteroarene. The TRPswitch compounds enable reversible, repeatable, and nearly quantitative light-induced activation and deactivation of the vertebrate TRPA1 channel with violet and green light, respectively. The utility of TRPswitch compounds was demonstrated in larval zebrafish hearts exogenously expressing zebrafish Trpa1b, where the heartbeat could be controlled using TRPswitch and light. Therefore, TRPA1/TRPswitch represents a novel step-function chemo-optogenetic system with a unique combination of high conductance, high efficiency, activity against an unmodified vertebrate channel, and capacity for bidirectional optical switching. This chemo-optogenetic system will be particularly applicable in systems where a large depolarization current is needed or sustained channel activation is desirable.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.