Evidence map›Paper›PMID 32964916›Full record

ArticleBioscience reports2020

MicroRNA-223 decreases cell proliferation, migration, invasion, and enhances cell apoptosis in childhood acute lymphoblastic leukemia via targeting Forkhead box O 1.

Chunyu Li, Tana Zhao, Lei Nie, Yanhong Zou, Quan Zhang

RetractedOpen access · goldAbstract readRetracted Publication
PubMed Full text at PMC Publisher
In one paragraph

Article in Bioscience reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It has been retracted, and should not be counted. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.8field-weighted citation impact, top 30% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 12 citations in OpenAlex.

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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Chunyu Li *Department of Pediatrics, The First Affiliated Hospital of Jiamusi University, No. 348 dexiang Street, Jiamusi City, Heilongjiang Province 154002, China.
Tana Zhao *Department of Pediatrics, The First Affiliated Hospital of Jiamusi University, No. 348 dexiang Street, Jiamusi City, Heilongjiang Province 154002, China.
Lei NieDepartment of Pediatrics, The First Affiliated Hospital of Jiamusi University, No. 348 dexiang Street, Jiamusi City, Heilongjiang Province 154002, China.
Yanhong ZouDepartment of Pediatrics, The First Affiliated Hospital of Jiamusi University, No. 348 dexiang Street, Jiamusi City, Heilongjiang Province 154002, China.
Quan ZhangDepartment of Gastroenterology, Jiamusi Central Hospital, No. 256, Zhongshan Street, Xiangyang District, Jiamusi City, Heilongjiang Province 154002, China.
First Affiliated Hospital of Jiamusi University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveAcute lymphoblastic leukemia (ALL) is a frequent malignancy in childhood. The present study was aimed to investigate the effect of miR-223 in ALL and its underlying molecular mechanisms.

methodsThe mRNA expression of miR-223 and FOXO1 was detected by qRT-RCR in ALL children. The correlation between miR-223 and clinical indexes of ALL was determined. CCRF-CEM and NALM-6 cells were transfected with miR-223 mimic and miR-223 inhibitor, respectively. The proliferation, apoptosis, invasion and migration of CCRF-CEM and NALM-6 cells were measured by MTT, flow cytometry and transwell assay. The protein expression of FOXO1 was detected by Western blot. Additionally, dual-luciferase reporter and RNA pull-down assay were performed to investigate the target gene of miR-223 and validate their targeting relationship.

resultsThe mRNA expression of miR-223 was markedly down-regulated in ALL, but FOXO1 was up-regulated. The protein expression of FOXO1 was highly expressed in CCRF-CEM and NALM-6 cells. The expression of miR-223 was related to WBC, PLT, RBC and risk stratification. Overexpression of miR-223 not only inhibited cell proliferation, migration and invasion, but also induced cell apoptosis. Importantly, FOXO1 was a target gene of miR-223 in ALL cells. Silencing of FOXO1 reversed the effects of miR-223 inhibitor on cell proliferation, migration, invasion and apoptosis in ALL.

conclusionsmiR-223 could inhibit cell proliferation, migration and invasion, and promote apoptosis by targeting FOXO1 in ALL.

Indexed as

ApoptosisCell MovementCell ProliferationAdolescentCase-Control StudiesCell Line, TumorChildFemaleForkhead Box Protein O1Gene Expression Regulation, LeukemicHumansMaleMicroRNAsNeoplasm InvasivenessPrecursor Cell Lymphoblastic Leukemia-LymphomaSignal TransductionForkhead Box Protein O1FOXO1 protein, humanMicroRNAsMIR223, humanacute lymphoblastic leukemiacell apoptosiscell proliferationFOXO1migration and invasionmiR-223

Identifiers

PMID32964916
PMCPMC7538682
OpenAlexW3088565509

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.