ArticleRedox biology2020
High-dose folic acid supplementation results in significant accumulation of unmetabolized homocysteine, leading to severe oxidative stress in Caenorhabditis elegans.
Article in Redox biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 43 citations in OpenAlex.
- Pro-Carcinogenic Implications of Folic Acid Supplementation During Chemically Induced Cutaneous Squamous Cell Carcinoma in Mice.Journal of biochemical and molecular toxicology · 2026Article
- Excess folate elevates formaldehyde genotoxicity in cell lines but not in mice or humans.EMBO molecular medicine · 2026Article
- Intake of B vitamins and their circulating levels in relation to incident stroke in women and men: Findings from two national prospective cohorts in the United States.American journal of preventive cardiology · 2026Article
- Effects of Vitamin B6 and Folic Acid on Cardiometabolic Biomarkers and Cardiac Oxidative Stress in Homocysteine-Loaded Rats.Biomedicines · 2026Article
- Effects of Dietary Folic Acid Supplementation on Productive Performance, Egg Quality, Flavor, and Folate Species Deposition in Laying Hens.Animals : an open access journal from MDPI · 2026Article
- High-dose folic acid supplement is associated with increased cardiovascular risk in rheumatoid arthritis patients on methotrexate.Therapeutic advances in musculoskeletal disease · 2026Article
- High-dose folic acid use and cancer risk in women who have given birth: A register-based cohort study.Epilepsia · 2025Article
- The association of metabolic profile of folate with diabetic kidney disease: evidence from 2011-2020 cycles of the NHANES.Renal failure · 2024Article
- Methylenetetrahydrofolate reductase (MTHFR) polymorphisms in andrology-a narrative review.Translational andrology and urology · 2024Review
- Folic acid improved memory and learning function in a rat model of neuroinflammation induced by lipopolysaccharide.Inflammopharmacology · 2024Article
- Associations of Folate/Folic Acid Supplementation Alone and in Combination With Other B Vitamins on Dementia Risk and Brain Structure: Evidence From 466 224 UK Biobank Participants.The journals of gerontology. Series A, Biological sciences and medical sciences · 2024Article
- Impact of l-citrulline on nitric oxide signaling and arginase activity in hypoxic human pulmonary artery endothelial cells.Pulmonary circulation · 2023Article
- Article
- Molecular pathways and nutrigenomic review of insulin resistance development in gestational diabetes mellitus.Frontiers in nutrition · 2023Review
- Higher Concentrations of Folic Acid Cause Oxidative Stress, Acute Cytotoxicity, and Long-Term Fibrogenic Changes in Kidney Epithelial Cells.Chemical research in toxicology · 2022Article
- High Dietary Folic Acid Intake Is Associated with Genomic Instability in Peripheral Lymphocytes of Healthy Adults.Nutrients · 2022Observational
- Review
- Excessive Folic Acid Mimics Folate Deficiency in Human Lymphocytes.Current issues in molecular biology · 2022Article
- Folic acid, either solely or combined with L-citrulline, improves NO signaling and ameliorates chronic hypoxia-induced pulmonary hypertension in newborn pigs.Physiological reports · 2021Article
- CRIF1 Deficiency Increased Homocysteine Production by Disrupting Dihydrofolate Reductase Expression in Vascular Endothelial Cells.Antioxidants (Basel, Switzerland) · 2021Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Using Caenorhabditis elegans as a model animal, we evaluated the effects of chronical supplementation with high-dose folic acid on physiological events such as life cycle and egg-laying capacity and folate metabolism. Supplementation of high-dose folic acid significantly reduced egg-laying capacity. The treated worms contained a substantial amount of unmetabolized folic acid and exhibited a significant downregulation of the mRNAs of cobalamin-dependent methionine synthase reductase and 5,10-methylenetetrahydrofolate reductase. In vitro experiments showed that folic acid significantly inhibited the activity of cobalamin-dependent methionine synthase involved in the metabolism of both folate and methionine. In turn, these metabolic disorders induced the accumulation of unmetabolized homocysteine, leading to severe oxidative stress in worms. These results were similar to the phenomena observed in mammals during folate deficiency.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.