Evidence map›Paper›PMID 32960567›Full record

ArticleACS chemical neuroscience2020

Fast Purification of Recombinant Monomeric Amyloid-β from

Amberley D Stephens, Meng Lu, Ana Fernandez-Villegas, Gabriele S Kaminski Schierle

Open access · hybridAbstract read
In one paragraph

Article in ACS chemical neuroscience, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.5field-weighted citation impact, top 35% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 7 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Intracellular Aβ42 Aggregation Leads to Cellular Thermogenesis.Journal of the American Chemical Society · 2022
    Article
  5. Article
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Amberley D StephensChemical Engineering and Biotechnology, University of Cambridge, Philippa Fawcett Drive, Cambridge, CB3 0AS, United Kingdom.ORCID 0000-0002-7303-6392
Meng LuChemical Engineering and Biotechnology, University of Cambridge, Philippa Fawcett Drive, Cambridge, CB3 0AS, United Kingdom.
Ana Fernandez-VillegasChemical Engineering and Biotechnology, University of Cambridge, Philippa Fawcett Drive, Cambridge, CB3 0AS, United Kingdom.
Gabriele S Kaminski SchierleChemical Engineering and Biotechnology, University of Cambridge, Philippa Fawcett Drive, Cambridge, CB3 0AS, United Kingdom.ORCID 0000-0002-1843-2202
University of Cambridge · GB

Funding

Biotechnology and Biological Sciences Research Council BB/H023917/1Medical Research Council G0902243Medical Research Council MR/K02292X/1Wellcome TrustWellcome Trust 065807/Z/01/ZWellcome Trust 203249/Z/16/Z
6 · The paper itself

Abstract

The Alzheimer's disease related peptide, Amyloid-beta (Aβ)1-40 and 1-42, has proven difficult to be purified as a recombinant monomeric protein due its expression in

Indexed as

Alzheimer DiseaseEscherichia coliAmyloid beta-PeptidesAnimalsPeptide FragmentsRecombinant ProteinsAmyloid beta-PeptidesPeptide FragmentsRecombinant Proteinsamyloidarctic mutantAβ40Aβ42dye labelingE22Gfluorescenceinclusion bodiesion exchange chromatographymaleimidemCherry

Identifiers

PMID32960567
PMCPMC7581289
OpenAlexW3089056835

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.