Evidence map›Paper›PMID 32959190›Full record

ArticleBiotechnology letters2021

Enrichment and detection of circulating tumor cells by immunomagnetic beads and flow cytometry.

Lei Hu, Xueran Chen, Meng Chen, Jinman Fang, Jinfu Nie, Haiming Dai

Abstract read
PubMed Publisher
In one paragraph

Article in Biotechnology letters, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.6field-weighted citation impact, top 30% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 12 citations in OpenAlex.

  1. Review
  2. An Immunomagnetic Bead-Based Sandwich ELISA for the Detection ofAnimals : an open access journal from MDPI · 2026
    Article
  3. Article
  4. Review
  5. Article
  6. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Lei HuAnhui Province Key Laboratory of Medical Physics and Technology, Institute of Health & Medical Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, 230031, China.
Xueran ChenAnhui Province Key Laboratory of Medical Physics and Technology, Institute of Health & Medical Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, 230031, China.
Meng ChenAnhui Province Key Laboratory of Medical Physics and Technology, Institute of Health & Medical Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, 230031, China.
Jinman FangAnhui Province Key Laboratory of Medical Physics and Technology, Institute of Health & Medical Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, 230031, China.
Jinfu NieAnhui Province Key Laboratory of Medical Physics and Technology, Institute of Health & Medical Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, 230031, China. jinfunie@163.com.
Haiming DaiAnhui Province Key Laboratory of Medical Physics and Technology, Institute of Health & Medical Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, 230031, China. daih@cmpt.ac.cn.ORCID http://orcid.org/0000-0002-0484-7407
Chinese Academy of Sciences · CNUniversity of Science and Technology of China · CN

Funding

National Natural Science Foundation of China 21703254National Natural Science Foundation of China 21772201National Natural Science Foundation of China 81572948The co-operative grant from Anhui Medical University and Center of Medical Physics and Technology LHJJ202007
6 · The paper itself

Abstract

objectiveThe purpose of the article is to establish a quick enrichment and detection method using immunomagnetic beads and flow cytometry to analyze circulating tumor cells (CTCs) in the peripheral blood.

resultsAfter incubation with CD326-PE and CD45-APC antibodies, more than 60% MCF7 cells in M-Buffer could be detected while less than 10% of the same cells could be detected by flow cytometry (FCM) if spiked into blood. However, in combination with CD326 and CD45 immunomagnetic beads, detection rate of MCF7 cells in blood reached 57%. For circulating tumor cells, enrichment by CD326 and CD45 immunomagnetic beads improve the detection rate from nearly undetectable to more than 24.14%.

conclusionsLive CTCs in peripheral blood can be effectively and sensitively detected by using a combination of immunomagnetic beads (CD45 and CD326) and flow cytometry.

Indexed as

AgedEpithelial Cell Adhesion MoleculeFemaleFlow CytometryFluorescent Antibody TechniqueHumansImmunomagnetic SeparationLeukocyte Common AntigensMaleMCF-7 CellsMiddle AgedNeoplasmsNeoplastic Cells, CirculatingEpithelial Cell Adhesion MoleculeLeukocyte Common AntigensPTPRC protein, humanCirculating tumor cellsFlow cytometryFluorescence antibodiesImmunomagnetic beads

Identifiers

PMID32959190
OpenAlexW3087520194

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.