ArticleNature methods2020
Plasmonic scattering imaging of single proteins and binding kinetics.
Article in Nature methods, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 74 papers.
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Who cites it
74 citing papers in PubMed.
- Characterizing Variations in Binding Kinetics of Affinity Biosensors: A Boundary-Layer and Site-Quality Approach.ACS omega · 2026Article
- Integrated GNPS Molecular Networking and Network Pharmacology Uncover Methylophiopogonanone B as a Novel Anti-Inflammatory Agent From Polygonatum cyrtonema Hua Targeting the SRC-PI3K-Akt Pathway.Biomedical chromatography : BMC · 2026Article
- Illuminating the Interface: In Situ Optical Insights Into Two-Electron ORR Pathways for HAdvanced materials (Deerfield Beach, Fla.) · 2026Review
- Surface Plasmon Resonance Imaging and Microscopy Modalities for Information-Rich, Label-Free Analysis of Biomolecular Interactions and Disease Biomarkers.Chemical & biomedical imaging · 2026Review
- Evaluating Antibody Quality via Simultaneous Size and Charge Measurement with Single Protein Oscillators.Analytical chemistry · 2026Article
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- Rapid Filtration Allows Ultrafast Measurement of Antibody and Nanoparticle Capture in Porous Matrices: Implications for Lateral Flow Immunoassays and Membrane Chromatography.ACS applied nano materials · 2026Article
- The Fantastic Single-Molecule Techniques.Chemical & biomedical imaging · 2026Review
- IR-AMES uncovers structure and composition of Alzheimer's tau oligomers.bioRxiv : the preprint server for biology · 2026Article
- Label-free mass and size characterization of few-kDa biomolecules by hierarchical vision transformer augmented nanofluidic scattering microscopy.Nature communications · 2026Article
- Optical imaging of the intrinsic adsorption kinetics in single zeolite nanoparticles.Nature communications · 2026Article
- Extracellular Vesicle-Based Biomarkers in Spinal Cord Injury: A State-of-the-Art Review on Diagnostic and Prognostic Advances.International journal of molecular sciences · 2026Review
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- Meta-amplified dark-field interferometric scattering microscopy.Nature communications · 2026Article
- Wide-Field Digital Surface-Enhanced Raman Scattering: Quantitative Single-Molecule Detection with High Sensitivity and Throughput.Chemical & biomedical imaging · 2025Article
- Obtain substance of anti-glioblastoma from Erigeron breviscapus through fragment-based target research (FBTR): An efficient strategy for pharmacology investigation and optimization of natural products.Journal of pharmaceutical analysis · 2025Article
- Quantum relaxometry for detecting biomolecular interactions with single NV centers.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Quantitative, Label-Free Mapping of Cell Force Dynamics.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- Label-Free Single-Molecule Immunoassay.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Plasmonic DNA-Barcoded Virion Nano-Oscillators for Multiplexed Quantification of Small-Molecule Binding Kinetics to Membrane Proteins.Angewandte Chemie (International ed. in English) · 2025Article
14 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors.
Funding
Abstract
Measuring the binding kinetics of single proteins represents one of the most important and challenging tasks in protein analysis. Here we show that this is possible using a surface plasmon resonance (SPR) scattering technique. SPR is a popular label-free detection technology because of its extraordinary sensitivity, but it has never been used for imaging single proteins. We overcome this limitation by imaging scattering of surface plasmonic waves by proteins. This allows us to image single proteins, measure their sizes and identify them based on their specific binding to antibodies. We further show that it is possible to quantify protein binding kinetics by counting the binding of individual molecules, providing a digital method to measure binding kinetics and analyze heterogeneity of protein behavior. We anticipate that this imaging method will become an important tool for single protein analysis, especially for low volume samples, such as single cells.
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